Owing to their high spatiotemporal precision and adaptability to different host cells, organ-on-a-chip systems are showing great promise in drug discovery, developmental biology studies and disease modeling. However, many current micro-engineered biomimetic systems are limited in technological application because of culture media mixing that does not allow direct incorporation of techniques from stem cell biology, such as organoids. Here, we describe a detailed alternative method to cultivate millimeter-scale functional vascularized tissues on a biofabricated platform, termed ‘integrated vasculature for assessing dynamic events’, that enables facile incorporation of organoid technology. Utilizing the 3D stamping technique with a synthetic polymeric elastomer, a scaffold termed ‘AngioTube’ is generated with a central microchannel that has the mechanical stability to support a perfusable vascular system and the self-assembly of various parenchymal tissues. We demonstrate an increase in user familiarity and content analysis by situating the scaffold on a footprint of a 96-well plate. Uniquely, the platform can be used for facile connection of two or more tissue compartments in series through a common vasculature. Built-in micropores enable the studies of cell invasion involved in both angiogenesis and metastasis. We describe how this protocol can be applied to create both vascularized cardiac and hepatic tissues, metastatic breast cancer tissue and personalized pancreatic cancer tissue through incorporation of patient-derived organoids. Platform assembly to populating the scaffold with cells of interest into perfusable functional vascularized tissue will require 12–14 d and an additional 4 d if pre-polymer and master molds are needed.

由于其高时空精度和对不同宿主细胞的适应性，芯片上器官系统在药物发现、发育生物学研究和疾病建模方面显示出巨大的前景。然而，许多当前的微工程仿生系统在技术应用方面受到限制，因为培养基混合不允许直接结合来自干细胞生物学的技术，例如类器官。在这里，我们描述了一种在生物制造平台上培养毫米级功能性血管化组织的详细替代方法，称为“用于评估动态事件的集成脉管系统”，该方法可以轻松整合类器官技术。利用合成聚合物弹性体的 3D 冲压技术，一种名为“AngioTube”的支架由中央微通道生成，该微通道具有机械稳定性以支持可灌注的血管系统和各种实质组织的自组装。我们通过将脚手架放置在 96 孔板的足迹上来证明用户熟悉度和内容分析的增加。独特的是，该平台可用于通过公共脉管系统轻松连接两个或多个组织隔室。内置微孔能够研究涉及血管生成和转移的细胞侵袭。我们描述了如何通过纳入患者衍生的类器官，将该协议应用于创建血管化的心脏和肝脏组织、转移性乳腺癌组织和个性化胰腺癌组织。