Methanosarcina sp. strain MSH10X1, a psychrotrophic methanogen, was isolated from sub-seafloor methane hydrate deposits of Krishna Godavari Basin on India's east coast. The strain could grow from 5 to 40 °C following all three i.e. methylotrophic, acetoclastic, and hydrogenotrophic modes of methanogenesis utilizing different substrates like methanol, trimethylamine, H₂/CO₂ (80/20), acetate, valerate, isobutyrate, isopropanol, and isobutanol. The genome sequencing and analysis of this strain revealed a circular chromosome of 3,557,383 bp length having 42.47 mol% G + C content, which consisted of 3110 coding genes, 58 tRNA genes, and 3 rRNA operons. The KEGG analysis highlighted the presence of genes responsible for all three modes of methanogenesis. The presence of genes like mtaB, mtaC, and mttB in the genome provided evidence for possible adaptation of strain MSH10X1 in the deep sea's low-temperature conditions.

甲烷八叠球菌属 菌株 MSH10X1 是一种嗜冷产甲烷菌，是从印度东海岸 Krishna Godavari 盆地的海底甲烷水合物沉积物中分离出来的。该菌株可以在使用不同底物（如甲醇、三甲胺、H ₂ /CO _2）的所有三种（即甲基营养型、乙酰碎屑型和氢营养型产甲烷模式）下从 5 到 40 °C 生长(80/20)、醋酸盐、戊酸盐、异丁酸盐、异丙醇和异丁醇。该菌株的基因组测序和分析揭示了一条长 3,557,383 bp 的环状染色体，其 G+C 含量为 42.47 mol%，由 3110 个编码基因、58 个 tRNA 基因和 3 个 rRNA 操纵子组成。KEGG 分析强调了负责所有三种产甲烷模式的基因的存在。基因组中 mtaB、mtaC 和 mttB 等基因的存在为菌株 MSH10X1 在深海低温条件下的可能适应提供了证据。