Maternal deprivation (MD) is known to be related to long-term changes that could influence the onset of psychiatric disorders. Studies have demonstrated that early life stress makes the cells in the brain more susceptible to subsequent stressors. To test it, we used an animal model of MD conducted from postnatal day (PND) 1 to 10. Deprived and non-deprived rats (control) were randomized to receive or not lipopolysaccharide (LPS) at 5 mg/kg on PND 50. The behavior and glial cells activation were evaluated in all groups from 51 to 53 PND. There was an increase in the immobility time in the MD and MD+LPS groups. The spontaneous locomotor activity was not changed between groups. We found elevated ionized calcium-binding adapter molecule 1 (Iba-1)-positive cells levels in the control+LPS and MD+LPS groups. In the MD+LPS group, it was found an increase in Iba-positive cells compared to the MD+sal group. The glial fibrillary acidic protein (GFAP)-positive cells were also increased in the MD+LPS, compared to control+sal, control+LPS, and MD+sal groups. Immune challenge by LPS in late adolescence, which was subjected to MD, did not influence the depressive-like behavior but exerted a pronounced effect in the microglial activation and astrocyte atrophy.

中文翻译：

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早期生活压力和免疫挑战对青春期晚期大鼠行为和神经胶质细胞改变的影响

众所周知，母亲剥夺 (MD) 与可能影响精神疾病发作的长期变化有关。研究表明，早年的生活压力会使大脑中的细胞更容易受到后续压力源的影响。为了对其进行测试，我们使用了从出生后第 1 天 (PND) 到第 10 天进行的 MD 动物模型。剥夺和非剥夺大鼠（对照）随机接受或不接受 PND 50 时 5 mg/kg 的脂多糖 (LPS)。在 51 至 53 PND 的所有组中评估行为和神经胶质细胞活化。MD 和 MD+LPS 组的不动时间有所增加。各组之间的自发运动活动没有变化。我们发现在对照组 + LPS 和 MD + LPS 组中离子化钙结合衔接分子 1 (Iba-1) 阳性细胞水平升高。在MD+LPS组中，发现与 MD+sal 组相比，Iba 阳性细胞有所增加。与对照+sal、对照+LPS 和MD+sal 组相比，MD+LPS 中的神经胶质纤维酸性蛋白(GFAP) 阳性细胞也有所增加。在经历了 MD 的青春期晚期 LPS 的免疫挑战不影响抑郁样行为，但对小胶质细胞激活和星形胶质细胞萎缩产生显着影响。