Purpose

Malignant glioma cells are generally insensitive to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis. In this research, we designed a system containing mitoxantrone (MTX) which is loaded into poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) in order to increase sensitivity of glioblastoma cancer cell to TRAIL plasmid.

Methods

PLGA NPs were prepared using a water-in-oil-in-water (W/O/W) double emulsification and solvent evaporation technique and characterized. Synergistic cytotoxicity effect was assessed by both MTT and annexin V-FITC and PI analysis against GL-261 cancer cell line.

Result

The combination treatment with PLGA-MTX and TRAIL plasmid showed more cytotoxic effect on GL-261 cancer cell line (cell viability = 16%) compared to MTX-PLGA alone (cell viability 38%) and TRAIL alone (cell viability = 87%).

Conclusion

It concluded that PLGA-MTX can be considered a potential agent to sensitize glioblastoma cancer cell to TRAIL.

中文翻译：

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装载米托蒽醌的PLGA纳米颗粒可提高胶质母细胞瘤癌细胞对TRAIL诱导的细胞凋亡的敏感性

目的

恶性神经胶质瘤细胞通常对TRAIL（肿瘤坏死因子相关的凋亡诱导配体）诱导的凋亡不敏感。在这项研究中，我们设计了一个包含米托蒽醌（MTX）的系统，该系统被装载到聚乳酸-乙醇酸（PLGA）纳米颗粒（NPs）中，以增加胶质母细胞瘤癌细胞对TRAIL质粒的敏感性。

方法

PLGA NP使用水包油包水（W / O / W）双重乳化和溶剂蒸发技术制备并进行了表征。通过MTT和膜联蛋白V-FITC和PI分析评估针对GL-261癌细胞系的协同细胞毒性作用。

结果

与单独的MTX-PLGA（细胞活力38％）和单独的TRAIL（细胞活力= 87％）相比，PLGA-MTX和TRAIL质粒的联合治疗对GL-261癌细胞系（细胞活力= 16％）显示出更大的细胞毒性作用。

结论

结论是，PLGA-MTX可以被认为是使胶质母细胞瘤癌细胞对TRAIL敏感的潜在药物。