The feasibility and performance of continuous hydrogen production from herbal wastewater via anaerobic fermentation was investigated at different organic loading rates (OLRs) (from 12 to 96 kg chemical oxygen demand [COD]·L⁻¹·day⁻¹) in diatomite-immobilized sludge reactor and suspended sludge reactor. Results indicated that the diatomite-immobilized sludge reactor presented more efficient and stable performance than suspended sludge reactor in terms of hydrogen production rate. In diatomite-immobilized sludge reactor, the highest hydrogen production rate of 11.17 ± 0.45 mmol·L⁻¹·h⁻¹ was achieved at the optimum OLR of 72-kg COD·L⁻¹·day⁻¹. The metabolic route of acetate acid and butyrate acid production at low OLR (from 12- to 40-kg COD·L⁻¹·day⁻¹) was modified to be ethanol production by an increase in OLR to 56 and 72-kg COD·L⁻¹·day⁻¹, which was reflected by the distribution of bacterial community. The maximum energy recovery efficiency by dual production of hydrogen and ethanol was determined to be 31.2 kJ·L⁻¹·h⁻¹ at OLR of 72-kg COD·L⁻¹·day⁻¹.

中文翻译：

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硅藻土固定污泥厌氧发酵从草药废水中制氢

研究了在不同有机负荷率 (OLR)（从 12 到 96 kg 化学需氧量 [COD]·L ^-1 ·day ^-1）在硅藻土固定污泥中通过厌氧发酵从草药废水中连续制氢的可行性和性能反应器和悬浮污泥反应器。结果表明，硅藻土固定化污泥反应器在产氢率方面表现出比悬浮污泥反应器更高效、更稳定的性能。^{在硅藻土固定化污泥反应器中，在 72-kg COD·L -1} ·day ^-1的最佳 OLR 下实现了11.17 ± 0.45 mmol·L ^-1 ·h ^-1的最高产氢率. 通过将 OLR 增加到 56 和 72-kg COD·，将低 OLR（从 12- 到 40-kg COD·L ^-1 ·day ^-1 ）下产生乙酸和丁酸的代谢途径修改为乙醇生产L ^-1 ·day ^-1，这反映在细菌群落的分布上。在72-kg COD·L ^-1 ·day ^-1的OLR下，氢气和乙醇的双重生产的最大能量回收效率被确定为31.2 kJ·L ^-1 ·h ^-1。