Objective:

Dentin hypersensitivity (DH) is a common oral disease with approximately 41.9% prevalence. Reconstruction of dental hard tissues is the preferred treatment for relieving DH. Here, we applied biomineralization method using oligopeptide simulating cementum protein 1 (CEMP1) to regenerate hard tissues on demineralized dentin.

Methods:

The self-assembly and biomineralization property of the oligopeptide were detected by scanning electron microscopy (SEM), circular dichroism spectroscopy, and transmission electron microscopy. Oligopeptide’s binding capacity to demineralized dentin was evaluated by SEM and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Remineralization was characterized using SEM, ATR-FTIR, X-ray diffraction, and nanoindentation. Oligopeptide’s biocompatibility was evaluated using periodontal ligament cells.

Results:

Oligopeptides self-assembled into nano-matrix and templated mineral precursor formation within 24 h. Moreover, oligopeptide nano-matrix bound firmly on demineralized dentin and resisted water rinsing. Then, bound nano-matrix served as a template to initiate nucleation and transformation of hydroxyapatite on demineralized dentin. After 96 h, oligopeptide nano-matrix regenerated an enamel-like tissue layer with a thickness of 15.35 μm, and regenerated crystals occluded dentin tubules with a depth of 31.27 μm. Furthermore, the oligopeptide nano-matrix had good biocompatibility when co-cultured with periodontal ligament cells.

Conclusions:

This biomimetic oligopeptide simulating CEMP1 effectively induced remineralization and reconstructed hard tissues on demineralized dentin, providing a potential biomaterial for DH treatment.

中文翻译：

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仿生寡肽通过矿化形成牙釉质样组织和牙本质小管闭塞治疗牙本质过敏

客观的：

牙本质过敏症（DH）是一种常见的口腔疾病，患病率约为 41.9%。牙齿硬组织重建是缓解DH的首选治疗方法。在这里，我们应用生物矿化方法使用模拟牙骨质蛋白 1 (CEMP1) 的寡肽来再生脱矿牙本质上的硬组织。

方法：

通过扫描电子显微镜（SEM）、圆二色光谱和透射电子显微镜检测寡肽的自组装和生物矿化特性。通过 SEM 和衰减全反射傅里叶变换红外光谱 (ATR-FTIR) 评估寡肽与脱矿质牙本质的结合能力。使用 SEM、ATR-FTIR、X 射线衍射和纳米压痕表征再矿化。使用牙周膜细胞评估寡肽的生物相容性。

结果：

寡肽在 24 小时内自组装成纳米基质和模板矿物前体形成。此外，寡肽纳米基质牢固地结合在脱矿质牙本质上并抵抗水冲洗。然后，结合的纳米基质作为模板启动羟基磷灰石在脱矿质牙本质上的成核和转化。96 h后，寡肽纳米基质再生出一层厚度为15.35 μm的牙釉质样组织层，再生晶体封闭牙本质小管，深度为31.27 μm。此外，寡肽纳米基质在与牙周膜细胞共培养时具有良好的生物相容性。

结论：

这种模拟CEMP1的仿生寡肽有效诱导脱矿牙本质上的再矿化和重建硬组织，为DH治疗提供了潜在的生物材料。