The nutrient contents of Raphanus sativus L. (white radish) leaves (RSLs) and roots are known to have promising vital effects. We comparatively investigated the leaves and roots of R. sativus grown in Saudi Arabia to estimate the total phenol and flavonoid contents using the standard colorimetric methods. The antioxidant activity of RSLs and R. sativus roots (RSRs) were measured by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Both extracts’ cytotoxic activity was assessed by MTT assay against several human cancer cell lines (A549, HepG2, MDA-MB-231, and MCF-7). High-performance thin-layer chromatography was used to identify and quantify the biomarker compound rutin in both extracts. The total phenolic content in RSLs (125.3 mg of gallic acid equivalent [GAE]/g) was higher than that in roots (95.8 of GAE/g), whereas the flavonoid content (44.5 mg of quercetin equivalent [QE]/g) was almost double compared in RSR extract (24.4 of QE/g). Similarly, RSLs exhibited a higher antioxidative activity than RSRs in both DPPH (IC 50 216.8 vs 359.7 µg/mL) and ABTS (IC 50 326.7 vs 549 µg/mL) models. RSLs also demonstrated the highest antiproliferative efficiency against all cell lines, with IC 50 values of 217–453 µg/mL. The reversed-phase-high-performance thin-layer chromatography results showed the presence of rutin (5.2 µg/mg) only in RSLs. Our study indicates RSLs as a promising source of bioactive compounds compared with roots.

中文翻译：

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沙特阿拉伯生长的白萝卜（Raphanus sativus L.）叶片和根提取物中芦丁的抗氧化和抗癌活性比较以及HPTLC定量的芦丁

众所周知，萝卜（Raphanus sativus L。）（白萝卜）叶（RSLs）和根的营养成分具有重要的生命作用。我们通过标准比色法比较研究了在沙特阿拉伯种植的红景天的叶和根，以估算其总酚和类黄酮的含量。通过2,2'-叠氮基-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和1,1-二苯基-2-吡啶并肼基（DPPH）测定来测定RSL和茄根（RSR）根的抗氧化活性。 。两种提取物的细胞毒性活性均通过MTT分析针对几种人类癌细胞系（A549，HepG2，MDA-MB-231和MCF-7）进行了评估。高性能薄层色谱用于鉴定和定量两种提取物中的生物标志化合物芦丁。RSL中的总酚含量（125。3 mg没食子酸当量[GAE] / g）高于根部（95.8 GAE / g），而RSR提取物中的类黄酮含量（44.5 mg槲皮素当量[QE] / g）几乎是根的两倍（ 24.4 QE / g）。同样，在DPPH（IC 50 216.8 vs 359.7 µg / mL）和ABTS（IC 50 326.7 vs 549 µg / mL）模型中，RSL都比RSR具有更高的抗氧化活性。RSL还显示出对所有细胞系的最高抗增殖效率，IC 50值为217–453 µg / mL。反相高效薄层色谱结果表明，仅在RSL中存在芦丁（5.2 µg / mg）。我们的研究表明，与根相比，RSLs是一种有希望的生物活性化合物来源。QE / g的4）。同样，在DPPH（IC 50 216.8 vs 359.7 µg / mL）和ABTS（IC 50 326.7 vs 549 µg / mL）模型中，RSL都比RSR具有更高的抗氧化活性。RSL还显示出对所有细胞系的最高抗增殖效率，IC 50值为217–453 µg / mL。反相高效薄层色谱结果表明，仅在RSL中存在芦丁（5.2 µg / mg）。我们的研究表明，与根相比，RSLs是一种有希望的生物活性化合物来源。QE / g的4）。同样，在DPPH（IC 50 216.8 vs 359.7 µg / mL）和ABTS（IC 50 326.7 vs 549 µg / mL）模型中，RSL都比RSR具有更高的抗氧化活性。RSL还显示出对所有细胞系的最高抗增殖效率，IC 50值为217–453 µg / mL。反相高效薄层色谱结果表明，仅在RSL中存在芦丁（5.2 µg / mg）。我们的研究表明，与根相比，RSLs是一种有希望的生物活性化合物来源。反相高效薄层色谱结果表明，仅在RSL中存在芦丁（5.2 µg / mg）。我们的研究表明，与根相比，RSLs是一种有希望的生物活性化合物来源。反相高效薄层色谱结果表明，仅在RSL中存在芦丁（5.2 µg / mg）。我们的研究表明，与根相比，RSLs是一种有希望的生物活性化合物来源。