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Contribution of zinc solubilizing bacterial isolates on enhanced zinc uptake and growth promotion of maize ( Zea mays L.)
Folia Microbiologica ( IF 2.6 ) Pub Date : 2021-03-30 , DOI: 10.1007/s12223-021-00863-3
Himantika Upadhyay 1 , Saurabh Gangola 2 , Anita Sharma 1 , Arjun Singh 3, 4 , Damini Maithani 1 , Samiksha Joshi 5
Affiliation  

Zinc-solubilizing bacteria, namely Burkholderia cepacia and Acinetobacter baumannii (H1 and H3, respectively), able to grow in liquid basal medium supplemented with ZnO, Zn3 (PO4)2, and ZnSO4·7H2O (0.1%), showed plant growth promoting properties. The treatment of Acinetobacter baumannii (H3) solubilizes the ZnO (1.42 ppm), Zn3 (PO4)2 (1.15 ppm), and ZnSO4·7H2O (1.44 ppm).The maximum solubilization of ZnSO4·7H2O (1.42 ppm) was observed in Burkholderia cepacia (H1) after 15 days. Organic acids produced by the bacteria decreased the pH of the medium and helped in Zn solubilization. In pot experiment on maize, Burkholderia cepacia (H1) treatment significantly enhanced plant height and root length in the presence of ZnO (2%) added in 10 mL of inoculum in each pot. High-performance liquid chromatography (HPLC) analysis of maize root extract showed the presence of oxalic, maleic, tartaric, and fumaric acid after 60 days of the experiment. Bacterial treatments enhanced sugar and protein level in maize plants and were 55.2 and 42.55 µg/mL plant extract, respectively, under mixture of bacterial treatment. Hence, isolates H1 and H3 expressed highest potential throughout the experiments, as zinc solubilizers and plant growth-promoting strains. This study demonstrated that meticulous use of Zn-solubilizing bacterial strains could aid in enhanced plant growth and can be the potential bio-inoculants for biofortification of maize to overcome the problems of malnutrition.



中文翻译:

锌增溶细菌分离株对增强玉米锌吸收和促进生长的贡献(Zea mays L.)

溶锌细菌,即洋葱伯克霍尔德菌鲍曼不动杆菌(分别为 H1 和 H3),能够在补充有 ZnO、Zn 3 (PO 4 ) 2和 ZnSO 4 ·7H 2 O (0.1%) 的液体基础培养基中生长,显示出植物生长促进特性。鲍曼不动杆菌(H3)的处理使ZnO (1.42 ppm)、Zn 3 (PO 4 ) 2 (1.15 ppm)和ZnSO 4 ·7H 2 O (1.44 ppm)增溶。ZnSO 4 ·7H 2 O的最大增溶(1.42 ppm) 在15 天后洋葱伯克霍尔德菌(H1)。细菌产生的有机酸降低了培养基的 pH 值并有助于锌的溶解。在玉米盆栽试验中,洋葱伯克霍尔德菌(H1) 在每盆 10 mL 接种物中添加 ZnO (2%) 的情况下,(H1) 处理显着提高了植物高度和根长。玉米根提取物的高效液相色谱 (HPLC) 分析表明,实验 60 天后存在草酸、马来酸、酒石酸和富马酸。细菌处理提高了玉米植物中的糖和蛋白质水平,在细菌处理的混合下分别为 55.2 和 42.55 µg/mL 植物提取物。因此,分离株 H1 和 H3 在整个实验过程中表现出最高的潜力,作为锌增溶剂和植物生长促进菌株。这项研究表明,精心使用锌增溶细菌菌株可以帮助促进植物生长,并且可以成为玉米生物强化以克服营养不良问题的潜在生物接种剂。

更新日期:2021-03-30
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