In this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were separated by electrophoresis and isoelectric focusing, and zinc-containing protein spots were identified by tandem mass spectrometry. Organic zinc-containing diets modified total royal jelly protein content and altered the expression of five major royal jelly proteins (MRJP1, MRJP2, MRJP3, MRJP5, and MRJP8). While the diet with the lowest tested zinc content (Zn25) increased total royal jelly protein content and expression of MRJP1, diets with higher zinc contents (Zn50 and Zn75) decreased MRJP expression. Our results demonstrate that important proteins and metabolic processes are modified in royal jelly produced by honey bees supplemented with different concentrations of organic zinc source, affecting nutrition and maintenance of colonies.

在这项研究中，我们分析了意大利蜜蜂补充不同浓度的膳食有机锌（0、25、50 和 75 ppm）引起的蜂王浆蛋白质组改变。通过电泳和等电聚焦分离从蜂王浆中提取的蛋白质，并通过串联质谱法鉴定含锌蛋白质点。有机含锌饮食改变了蜂王浆总蛋白含量并改变了五种主要蜂王浆蛋白（MRJP1、MRJP2、MRJP3、MRJP5 和 MRJP8）的表达。虽然测试锌含量最低的饮食 (Zn25) 增加了蜂王浆总蛋白含量和 MRJP1 的表达，但锌含量较高的饮食 (Zn50 和 Zn75) 降低了 MRJP 的表达。