SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients’ samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis.

SARS-CoV-2 RNA 被确定为支持 COVID-19 检测能量区的关键因素。在此，我们开发了一种快速且非放大的纳米传感平台，用于检测人咽拭子样本中的 SARS-CoV-2 RNA。制备了金纳米粒子 (AuNP) 修饰的石墨烯场效应晶体管 (G-FET) 传感器，然后将互补的二氨基磷酸酯吗啉代寡核苷酸 (PMO) 探针固定在 AuNP 表面上。该传感器允许对 SARS-CoV-2 RdRp 进行高灵敏度测试，因为 PMO 不带电荷，从而导致背景信号较低。该方法不仅在 PBS (0.37 fM)、咽拭子 (2.29 fM) 和血清 (3.99 fM) 中呈现低检测限，而且在 2 分钟内实现了对 COVID-19 患者样本的快速响应。开发的纳米传感器能够分析 30 个真实临床样本的 RNA 提取物。结果表明，传感器可以区分健康人和感染者，这与 RT-PCR 结果（Kappa 指数 = 0.92）高度一致。此外，还明确区分了 SARS-CoV-2 RdRp 和 SARS-CoV RdRp。因此，我们相信这项工作为 COVID-19 诊断提供了一个令人满意的、有吸引力的选择。