Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm–2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm–2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.

中文翻译：

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初始粘附与蓝藻生物膜发育之间的关联

尽管实验室化验提供了有关海洋表面的防污效果和生物膜形成动力学的宝贵信息，但它们可能既费力又耗时。本研究旨在确定短时间粘附试验的潜力，以估计生物膜发育可能如何进行。使用玻璃和聚合物环氧树脂表面在不同的流体动力学条件下评估初始粘附和蓝藻生物膜的形成，并使用线性回归模型进行比较。对于初始粘附，与玻璃相比，聚合物环氧树脂表面与较少数量的粘附细胞显着相关（-1.27 × 105 个细胞.cm-2）。同样，在 185 转时，粘附细胞的数量（-1.16 × 105 个细胞.cm-2）明显低于 40 转时。这种趋势在生物膜发育过程中得以维持，并受到生物膜湿重、厚度、叶绿素 a 含量和结构的支持。结果表明粘附细胞数和生物膜细胞数之间存在显着相关性（r = 0.800，p < 0.001）。此外，第 42 天的生物膜细胞数量取决于初始粘附结束时的粘附细胞数量和流体动力学条件（R2 = 0.795，p < 0.001）。这些发现证明了初始粘附测定在估计海洋生物膜发育方面的巨大潜力。第 42 天的生物膜细胞数量取决于初始粘附结束时的粘附细胞数量和流体动力学条件 (R2 = 0.795, p < 0.001)。这些发现证明了初始粘附测定在估计海洋生物膜发育方面的巨大潜力。第 42 天的生物膜细胞数量取决于初始粘附结束时的粘附细胞数量和流体动力学条件 (R2 = 0.795, p < 0.001)。这些发现证明了初始粘附测定在估计海洋生物膜发育方面的巨大潜力。