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Functional long non-coding and circular RNAs in zebrafish
Briefings in Functional Genomics ( IF 4 ) Pub Date : 2021-03-01 , DOI: 10.1093/bfgp/elab014
Gyan Ranjan , Paras Sehgal , Disha Sharma , Vinod Scaria , Sridhar Sivasubbu

The utility of model organisms to understand the function of a novel transcript/genes has allowed us to delineate their molecular mechanisms in maintaining cellular homeostasis. Organisms such as zebrafish have contributed a lot in the field of developmental and disease biology. Attributable to advancement and deep transcriptomics, many new transcript isoforms and non-coding RNAs such as long noncoding RNA (lncRNA) and circular RNAs (circRNAs) have been identified and cataloged in multiple databases and many more are yet to be identified. Various methods and tools have been utilized to identify lncRNAs/circRNAs in zebrafish using deep sequencing of transcriptomes as templates. Functional analysis of a few candidates such as tie1-AS, ECAL1 and CDR1as in zebrafish provides a prospective outline to approach other known or novel lncRNA/circRNA. New genetic alteration tools like TALENS and CRISPRs have helped in probing for the molecular function of lncRNA/circRNA in zebrafish. Further latest improvements in experimental and computational techniques offer the identification of lncRNA/circRNA counterparts in humans and zebrafish thereby allowing easy modeling and analysis of function at cellular level.

中文翻译:

斑马鱼中的功能性长链非编码和环状 RNA

模型生物在了解新转录物/基因的功能方面的效用使我们能够描述它们在维持细胞稳态中的分子机制。斑马鱼等生物在发育和疾病生物学领域做出了很大贡献。由于进步和深度转录组学,许多新的转录亚型和非编码 RNA,如长链非编码 RNA (lncRNA) 和环状 RNA (circRNA) 已在多个数据库中被识别和编目,还有更多尚未被识别。各种方法和工具已被用于使用转录组的深度测序作为模板来识别斑马鱼中的 lncRNAs/circRNAs。对斑马鱼中一些候选物(如 tie1-AS、ECAL1 和 CDR1as)的功能分析为接近其他已知或新型 lncRNA/circRNA 提供了一个前瞻性轮廓。TALENS 和 CRISPRs 等新的遗传改变工具有助于探索斑马鱼中 lncRNA/circRNA 的分子功能。实验和计算技术的进一步最新改进提供了人类和斑马鱼中 lncRNA/circRNA 对应物的识别,从而允许在细胞水平上轻松建模和分析功能。
更新日期:2021-03-01
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