The thyroid hormones, thyroxine (T₄) and triiodothyronine (T₃), are required to regulate complex developmental processes in vertebrates and are highly sensitive to endocrine-disrupting compounds. Previous studies demonstrate that dioctyl sodium sulfosuccinate (DOSS), a common constituent of pharmaceuticals, cosmetics, and food products, disrupts canonical signaling of adipocyte differentiation by binding a nuclear hormone receptor in the same superfamily as thyroid hormone (TH) receptors. The present study was designed to determine whether DOSS is capable of disrupting TH signaling using the American bullfrog, Rana (Lithobates) catesbeiana—a cosmopolitan frog species that undergoes TH-dependent metamorphosis to transition from an aquatic tadpole to a terrestrial juvenile frog. Premetamorphic R. catesbeiana tadpoles were injected with 2 pmol/g body weight T₃ or 10 pmol/g body weight T₄ to induce precocious metamorphosis, then exposed for 48 h to environmentally or clinically relevant DOSS concentrations (0.5, 5, and 50 mg/L). Gene expression of three classical TH-responsive targets (thra, thrb, and thibz) was measured in tadpole liver and tail fin tissue through reverse transcription quantitative polymerase chain reaction (RT-qPCR). DOSS disrupted gene expression in liver and tail fin tissue at all three concentrations tested but the patterns of expression differed by tissue, gene transcript, and TH treatment status. To our knowledge, this is the first demonstration that DOSS can alter TH signaling. Further exploration into DOSS disruption of TH signaling is warranted, because exposure may affect other TH-dependent processes, such as salmon smoltification and perinatal human development.

甲状腺激素，甲状腺素（T ₄）和三碘甲甲状腺素（T ₃），需要调节脊椎动物的复杂发育过程，并且对破坏内分泌的化合物高度敏感。先前的研究表明，药物，化妆品和食品的常见成分磺基琥珀酸二辛酯钠（DOSS）通过与甲状腺激素（TH）受体同一个超家族中的核激素受体结合，破坏脂肪细胞分化的规范信号。本研究旨在确定DOSS是否能够使用美国牛蛙Rana（Lithobates）catesbeiana破坏TH信号传导-一种大都会蛙种，经历TH依赖性变态，从水生t过渡到陆生幼蛙。Premetamorphic牛蛙蝌蚪与2皮摩尔/ g体重Ť注射₃或10皮摩尔/ g体重Ť ₄诱导早熟变态，然后暴露48小时，环境或临床相关DOSS浓度（0.5,5和50mg / L）。三种经典TH响应靶标（thra，thrb和thibz的基因表达通过逆转录定量聚合酶链反应（RT-qPCR）在t的肝脏和尾鳍组织中测量）。在所有三种测试浓度下，DOSS均破坏了肝和尾鳍组织中的基因表达，但表达模式因组织，基因转录本和TH治疗状态而异。据我们所知，这是DOSS可以改变TH信号的第一个证明。由于接触可能会影响其他依赖TH的过程，例如鲑鱼糖化和围生期人类发育，因此有必要对DOSS破坏TH信号进行进一步的研究。