In recent years, n-butanol, a type of alcohol, has been widely used from the chemical industry to the food industry. In this study, toxic effects of n-butanol's different concentrations (10, 50, 250, 500, 750, 1,000, and 1,250 mg/L) in Zebrafish Danio rerio embryos and larvae were investigated. For this purpose, Zebrafish embryos were exposed to n-butanol in acute semistatic applications. Teratogenic effects such as cardiac edema, scoliosis, lordosis, head development abnormality, yolk sac edema, and tail abnormality were determined at different time intervals (24, 48, 72, 96, and 120 h). Additionally, histopathological abnormalities such as vacuole formation in brain tissue and necrosis in liver tissue were observed at high doses (500, 750, and 1,000 mg/L) in all treatment groups at 96 h. It was determined that heart rate decreased at 48, 72, and 96 h due to an increase in concentration. In addition, alcohol-induced eye size reduction (microphthalmia) and single eye formation (cyclopia) are also among the effects observed in our research findings. In conclusion, n-butanol has been observed to cause intense neurotoxic, teratogenic, and cardiotoxic effects in Zebrafish embryos and larvae.

中文翻译：

---

正丁醇对斑马鱼发育的致畸和神经毒性作用

近年来，正丁醇作为醇类的一种，从化工行业到食品行业都得到了广泛的应用。在这项研究中，正丁醇的不同浓度（10、50、250、500、750、1,000 和 1,250 mg/L）对斑马鱼斑马鱼的毒性作用研究了胚胎和幼虫。为此，斑马鱼胚胎在急性半静态应用中暴露于正丁醇。在不同的时间间隔（24、48、72、96 和 120 小时）确定致畸作用，如心脏水肿、脊柱侧凸、脊柱前凸、头部发育异常、卵黄囊水肿和尾部异常。此外，所有治疗组在高剂量（500、750 和 1,000 mg/L）96 h 时均观察到脑组织空泡形成和肝组织坏死等组织病理学异常。经测定，由于浓度增加，心率在 48、72 和 96 小时下降。此外，酒精引起的眼睛缩小（小眼症）和单眼形成（独眼）也是我们研究结果中观察到的影响之一。综上所述，