Cultured fibroblast cells, especially dermal cells, are used for various types of scientific research, particularly within the medical field. Desirable features of the cells include their ease of isolation, rapid cellular growth, and high degree of robustness. Currently, fibroblasts are mainly used to obtain pluripotent cells via a reprogramming process. Dermal fibroblasts, are particularly useful for gene therapies used for promoting wound healing or minimizing skin aging. In recent years, fibroblast transfection efficiencies have significantly improved. In order to introduce molecules (most often DNA or RNA) into cells, viral-based systems (transduction) or non-viral methods (transfection) that include physical/mechanical processes or lipid reagents may be used. In this article, we describe critical points that should be considered when selecting a method for transfecting fibroblasts. The most effective methods used for the transfection of fibroblasts include both viral-based and non-viral nucleofection systems. These methods result in a high level of transgene expression and are superior in terms of transfection efficacy and viability.

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当前与原代成纤维细胞转染相关的标准和缺陷

培养的成纤维细胞，尤其是真皮细胞，用于各种类型的科学研究，特别是在医学领域。细胞的理想特征包括它们易于分离、快速的细胞生长和高度的稳健性。目前，成纤维细胞主要用于通过重编程过程获得多能细胞。真皮成纤维细胞对于用于促进伤口愈合或减少皮肤老化的基因疗法特别有用。近年来，成纤维细胞转染效率显着提高。为了将分子（最常见的是 DNA 或 RNA）引入细胞，可以使用基于病毒的系统（转导）或非病毒方法（转染），包括物理/机械过程或脂质试剂。在本文中，我们描述了在选择转染成纤维细胞方法时应考虑的关键点。用于成纤维细胞转染的最有效方法包括基于病毒和非病毒核转染系统。这些方法导致高水平的转基因表达，并且在转染效力和活力方面更胜一筹。