Despite of physiological and toxicological relevance, the potential of androgens to influence fish lipid metabolism remains poorly explored. Here, brown trout primary hepatocytes were exposed to six concentrations (1 nM to 100 μM) of dihydrotestosterone (DHT) and testosterone (T), to assess changes in the mRNA levels of genes covering diverse lipid metabolic pathways. Acsl1, essential for fatty acid activation, was up-regulated by T and DHT, whereas the lipogenic enzymes FAS and ACC were up-regulated by the highest (100 μM) concentration of T and DHT, respectively. ApoA1, the major component of high-density lipoprotein (HDL), was down-regulated by both androgens. PPARγ, linked to adipogenesis and peroxisomal β-oxidation, was down-regulated by T and DHT, while Acox1–3I, rate-limiting in peroxisomal β-oxidation, was down-regulated by T. Fabp1, StAR and LPL were not altered. Our findings suggest that androgens may impact on lipid transport, adipogenesis and fatty acid β-oxidation and promote lipogenesis in fish liver.

尽管有生理和毒理学相关性，但仍未充分探索雄激素影响鱼脂质代谢的潜力。在这里，将褐鳟原代肝细胞暴露于六种浓度（1 nM至100μM）的二氢睾丸激素（DHT）和睾丸激素（T）中，以评估涵盖多种脂质代谢途径的基因的mRNA水平变化。T和DHT上调了脂肪酸激活所必需的Acsl1，而T和DHT的最高浓度（100μM）分别上调了脂肪酶FAS和ACC。高密度脂蛋白（HDL）的主要成分ApoA1被两种雄激素均下调。与脂肪形成和过氧化物酶体β-氧化有关的PPARγ被T和DHT下调，而过氧化物酶体β-氧化的限速Acox1–3I被T和DHT下调。StAR和LPL不变。我们的研究结果表明，雄激素可能会影响脂质转运，脂肪生成和脂肪酸β-氧化，并促进鱼肝中的脂肪生成。