Melanoma could be extremely lethal due to its high invasiveness and rapid proliferation, which causes thousands of deaths worldwide every year. Because of the resistance of melanoma to chemo/radio/immunotherapy, effective treatment of melanoma remains a clinically unsolved puzzle. Some efforts have been made to utilize ultrasound (US) to control the migration of melanoma cells, but the underlying mechanism is still unclear. Here, the impact of US-induced microbubble (MB) cavitation on the migration and cell cycle arrest of A375 melanoma cells was studied. To quantitatively explore the relationship between US-activated MB activity and the changes of A375 cellular response, inertial cavitation dose (ICD) was evaluated at varied acoustic driving pressures and MB concentrations. The pool data results showed that A375 cells’ migrating capacity slightly fluctuated till ICD reached a certain threshold (3000 V × μs) and declined rapidly afterwards as the ICD further increased. Similar trend was observed in terms of the changes of G1-phase cell ratio. These findings suggested that, with appropriately selected parameters, MB-mediated cavitation can be effectively utilized to suppress the migration of A375 cells by reducing the proportion of cells arrested in G1 phase.

黑色素瘤具有高度的侵袭性和迅速扩散的特性，因此具有极高的致死性，每年在世界范围内造成数千人死亡。由于黑素瘤对化学疗法/放射疗法/免疫疗法的抵抗力，有效治疗黑素瘤仍然是临床上尚未解决的难题。已经做出了一些努力来利用超声（US）来控制黑素瘤细胞的迁移，但是其潜在机制仍不清楚。在这里，研究了美国诱导的微泡（MB）空化对A375黑色素瘤细胞迁移和细胞周期停滞的影响。为了定量研究US激活的MB活性与A375细胞反应变化之间的关系，在不同的声学驱动压力和MB浓度下评估了惯性空化剂量（ICD）。池数据结果表明，直到ICD达到一定阈值（3000 V×μs）为止，A375细胞的迁移能力才有轻微波动，此后随着ICD的进一步增加而迅速下降。在G1期细胞比例的变化方面观察到相似的趋势。这些结果表明，与适当选择的参数，MB介导的气穴，可以有效地利用通过减少在G1期停滞细胞的比例以抑制A375细胞的迁移。