Regeneration is a widely spread process across the animal kingdom, including many species of marine crustaceans. It is strongly linked to hormonal cycles and, therefore, a great endpoint candidate for toxicology studies. We selected the amphipod Parhyale hawaiensis as test organism, already used in ecotoxicological studies and able to regenerate its body appendages. We are proposing a protocol to use the antenna regeneration as a toxicity endpoint. First, we evaluated differences in time of completion of regeneration in males and females after the amputation of one antenna of 6 months old animals. Then we compared the influence of different testing volumes in the regeneration process (100 and 5 mL). We used as testing substances, dimethyl sulfoxide (DMSO) and diflubenzuron, a chitin synthesis inhibitor. The most suitable protocol consisted of volumes of 5 mL in 12-well microplates, with 1 organism per well, 12 organisms per concentration (1:1 females/males) and test time duration of around 5 weeks. DMSO accelerated regeneration time with a NOEC of 0.06%. Diflubenzuron inhibited the time necessary to its completion with a NOEC of 0.32 μg L⁻¹. We conclude that the Parhyale hawaiensis antenna regeneration protocol proposed here is a potential tool in ecotoxicology, but more studies are required for its validation not only to verify its utility for testing chemicals but also environmental samples.

再生是整个动物界广泛传播的过程，包括许多海洋甲壳类动物。它与激素周期密切相关，因此是毒理学研究的理想终点。我们选择了两栖类夏威夷Parhyale作为测试有机体，已经在生态毒理学研究中使用，并且能够使其身体附件再生。我们正在提议一种使用天线再生作为毒性终点的方案。首先，我们评估了截肢6个月大动物的触角后，雄性和雌性再生完成时间的差异。然后，我们比较了不同测试量（100和5 mL）在再生过程中的影响。我们将二甲亚砜（DMSO）和几丁苯隆（几丁质合成抑制剂）用作测试物质。最合适的方案包括在12孔微孔板中加入5 mL的体积，每孔1种生物体，每种浓度12种生物体（1：1雌性/雄性），测试时间约5周。DMSO加快了再生时间，NOEC为0.06％。^-1。我们得出的结论是，此处提出的Parhyale hawaiensis天线再生协议是生态毒理学中的一种潜在工具，但是需要进行更多的研究才能对其进行验证，不仅是为了验证其在测试化学药品方面的效用，而且还应在验证环境样品的效用。