The phytotoxicity of soils under Wedelia trilobata has been described, but the dynamic between phytotoxic and metabolites in W. trilobata soil and the restriction posed by soil properties have been poorly addressed. In this study, the phytotoxic variation of W. trilobata soil in response to the changes in soil properties and soil metabolomics was determined at monthly intervals for 11 months using bioassays and metabolomic approaches. Strong variability in soil properties and obvious temporal variations in the soil phytotoxicity were observed over the 11-month period. Among the soil properties that were analyzed, soil organic matter, electrical conductivity, total nitrogen, and soil nutrients presented the most contribution in differentiating soils according to time of sampling and strength of phytotoxicity. Nonsterilized soil exerted stronger percentage inhibition of seed germination (IG) of lettuce, whereas the percentage inhibition of shoot height (SH) and root length (RL) of lettuce was higher in sterilized soil. Decline in the overall phytotoxicity was clearly observed in nonsterilized soil, suggesting the role of soil microbes in mediating the soil phytotoxic effect of W. trilobata, albeit the negative correlation. Putative metabolites contributing to the discrimination of soils according to time of sampling and strong correlation with percentage inhibition (%) of IG, SH, and RL were identified and discussed. Overall, the results showed that the phytotoxicity of soils under W. trilobata varied throughout the 11 months of study and can be restricted by the changes in soil properties.

中文翻译：

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三叶草土壤植物毒性与代谢产物的动态联系

已经描述了Wedelia trilobata下土壤的植物毒性，但W. trilobata土壤中植物毒性和代谢物之间的动态以及土壤特性带来的限制尚未得到很好的解决。在本研究中，W. trilobata的植物毒性变异土壤对土壤特性和土壤代谢组学变化的反应是使用生物测定和代谢组学方法在 11 个月内按月确定。在 11 个月的时间里，观察到土壤特性的强烈变化和土壤植物毒性的明显时间变化。在所分析的土壤特性中，土壤有机质、电导率、总氮和土壤养分对根据采样时间和植物毒性强度区分土壤的贡献最大。未消毒的土壤对生菜种子萌发 (IG) 的抑制百分比更强，而在已消毒的土壤中，对生菜芽高 (SH) 和根长 (RL) 的抑制百分比更高。在未消毒的土壤中清楚地观察到总体植物毒性的下降，W. trilobata，尽管呈负相关。根据采样时间和与 IG、SH 和 RL 的抑制百分比 (%) 的强相关性，确定并讨论了有助于区分土壤的推定代谢物。总体而言，结果表明，在整个 11 个月的研究中，三叶草下土壤的植物毒性发生了变化，并且可能受到土壤性质变化的限制。