Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.

2004年在阿根廷首次检测到李子痘病毒（PPV）。在根除最初的疫情，建立隔离区和严格控制苗圃植物之后，没有记录到疾病传播的迹象。在2018年春季，在门多萨省圣拉斐尔PPV隔离区一所房屋的花园中，蔷薇科“ Bridal Crown”（绣线菊）的观赏植物的叶子上观察到了由氯环引起的症状。通过DAS-ELISA对有症状的叶片进行PPV分析，结果为阳性。通过常规PCR和qRT-PCR证实了病毒的存在，并使用了绣线菊芽接T芽接枝接种的“ GF-305”生物指示剂植物。从绣线菊样品中扩增的一个243 bp的PCR片段被分离，测序并保存在GenBank（MN587037）中，该片段对应于PPV外壳蛋白基因的羧基末端区域。与其他PPV分离株的同源性为99％。RT-qPCR证实该病毒是先前在隔离区发现的PPV D株的分离株。我们的结果证实了PPV在新的观赏寄主“ Bridal Crown”上的自然感染，该寄主不属于李属，警告该物种可能是PPV的宿主和来源的潜在危险。