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Physiological and histopathological assessments of the susceptibility of different tomato ( Solanum lycopersicum ) cultivars to early blight disease
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-03-24 , DOI: 10.1007/s10658-021-02263-2
Nashwa M. A. Sallam , Heba-Alla S. AbdElfatah , Mona F. A. Dawood , Elhagag Ahmed Hassan , Mohamed S. Mohamed , Hadeel M. M. Khalil Bagy

The current research aims to evaluate the susceptibility of the tomato of the tomato (Solanum lycopersicum) cultivars 65,015 (CV1), Basma (CV2), Dalia (CV3), 320 (CV4), and 844 (CV5) to early blight disease caused by Alternaria solani, A. alternata, and Curvularia lunata under greenhouse conditions. All five cultivars were inoculated with the tested pathogenic fungi. The results showed that CV1 was highly resistant to early blight disease whereas CV5 was most susceptible to it. The causal pathogens decreased the total chlorophyll contents of the tomato cultivars and caused the accumulation of hydrogen peroxide, superoxide anions, and lipid peroxidation, having the greatest effect in CV5 followed by CV4 and the least effect in CV1. The pathogenic fungi also significantly stimulated the synthesis of ascorbic acid and reduced the production of glutathione, salicylic acid, soluble phenolic compounds, phenylalanine ammonialyase, and polyphenol oxidase in all the infected tomato cultivars except CV4 or CV5, with CV1 showing the greatest changes. Histopathological studies of the infection phases of A. solani and C. lunata on the highly resistant cultivar CV1 and the susceptible cultivar CV5 under the light microscope demonstrated that the leaves of CV1 were coated with thick epidermal hairs and glandular hairs and had relatively few stomata, whereas those of CV5 had a limited number of hairs and many stomata. Electron microscopy further showed that the fungal mycelia of A. solani and C. lunata easily attacked the open stomata of the hypersensitive cultivar CV5.



中文翻译:

番茄品种对早疫病敏感性的生理和组织病理学评价。

当前的研究旨在评估番茄(Solanum lycopersicum)品种中的番茄65,015(CV1),Basma(CV2),Dalia(CV3),320(CV4)和844(CV5)的易感性番茄早疫病菌链格孢弯孢菌叶斑病在温室条件下。所有五个品种均接种了测试的致病真菌。结果表明,CV1对早期枯萎病具有高度抗性,而CV5最容易感染这种疾病。因果病原体降低了番茄品种的总叶绿素含量,并导致了过氧化氢,超氧阴离子和脂质过氧化的积累,在CV5中影响最大,其次是CV4,而对CV1影响最小。除CV4或CV5以外,在所有感染的番茄品种中,病原性真菌还显着刺激了抗坏血酸的合成并减少了谷胱甘肽,水杨酸,可溶性酚类化合物,苯丙氨酸氨解酶和多酚氧化酶的产生,其中CV1的变化最大。牙周炎感染阶段的组织病理学研究在光学显微镜下,高抗性品种CV1和易感品种CV5上的A. solaniC. lunata表明,CV1的叶片被厚表皮毛和腺毛所覆盖,气孔相对较少,而CV5的叶片气孔有限。毛发和许多气孔。电子显微镜进一步显示,茄A. solaniC. lunata的真菌菌丝体容易攻击超敏品种CV5的开放气孔。

更新日期:2021-03-25
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