We have been using sandwich freezing of living yeast and bacteria followed by freeze-substitution for observing close-to-native ultrastructure of cells. Recently, sandwich freezing of glutaraldehyde-fixed cultured cells and human tissues have been found to give excellent preservation of ultrastructure of cells and tissues. These studies, however, have been conducted using a handmade sandwich freezing device and have been limited in a few laboratories. To spread the use of this method to other laboratories, we fabricated and commercialized a new sandwich freezing device. The new device is inexpensive, portable and sterilizable. It can be used to rapid-freeze viruses, bacteria, yeast, cultured cells and animal and human tissues to a depth of 0.2 mm if tissues are prefixed with glutaraldehyde. The commercial availability of this device will expand application of rapid freezing to wide range of biological materials.

中文翻译：

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用于在电子显微镜下快速冷冻病毒、细菌、酵母、培养细胞以及动物和人体组织的夹心冷冻装置

我们一直在使用活酵母和细菌的夹心冷冻，然后冷冻替代来观察接近天然的细胞超微结构。最近，已发现戊二醛固定的培养细胞和人体组织的夹心冷冻可以很好地保存细胞和组织的超微结构。然而，这些研究是使用手工制作的夹心冷冻装置进行的，并且仅限于少数实验室。为了将这种方法的使用推广到其他实验室，我们制造并商业化了一种新的夹心冷冻装置。新设备价格低廉、便携且可消毒。如果组织带有戊二醛前缀，它可用于将病毒、细菌、酵母、培养细胞以及动物和人体组织快速冷冻至 0.2 毫米的深度。