International Microbiology ( IF 3.1 ) Pub Date : 2021-03-23 , DOI: 10.1007/s10123-021-00173-1 Surendraraj Alagarsamy 1 , Sabeena Farvin K Habeebullah 1 , Faiza Al-Yamani 1
Sabkhas in Kuwait are unique hypersaline marine environments under-explored for bacterial community composition and bioprospecting. The 16S rRNA sequence analysis of 46 isolates with distinct morphology from two Kuwait sabkhas recovered 11 genera. Phylum Firmicutes dominated these isolates, and Bacillus (32.6%) was recovered as the dominant genera, followed by Halococcus (17.4%). These isolates were moderately halophilic, and some of them showed tolerance and growth at extreme levels of salt (20%), pH (5 and/or 11), and temperature (55 °C). A higher percentage of isolates harbored protease (63.0), followed by DNase (41.3), amylase (41.3), and lipase (32.6). Selected isolates showed antimicrobial activity against E. faecalis and isolated Halomonas shengliensis, and Idiomarina piscisalsi harbored gene coding for dNDP-glucose 4,6-dehydratase (Glu 1), indicating their potential to produce biomolecules with deoxysugar moieties. Palmitic acid or oleic acid was the dominant fatty acid, and seven isolates had some polyunsaturated fatty acids (linolenic or γ-linolenic acid). Interestingly, six isolates belonging to Planococcus and Oceanobacillus genus produced squalene, a bioactive isoprenoid molecule. Their content increased 30–50% in the presence of Terbinafine. The potential bioactivities and extreme growth conditions make this untapped bacterial diversity a promising candidate for future bioprospecting studies.
中文翻译:
中度嗜盐细菌的生物勘探潜力和科威特 sabkha 角鲨烯生产者的分离
科威特的 Sabkhas 是独特的高盐度海洋环境,细菌群落组成和生物勘探尚未充分探索。对来自两个科威特 sabkhas 的具有不同形态的 46 个分离物的 16S rRNA 序列分析恢复了 11 个属。厚壁菌门在这些分离株中占主导地位,芽孢杆菌(32.6%) 作为优势属被回收,其次是盐球菌(17.4%)。这些分离株具有中度嗜盐性,其中一些在盐 (20%)、pH (5 和/或 11) 和温度 (55 °C) 的极端水平下表现出耐受性和生长。较高百分比的分离株含有蛋白酶 (63.0),其次是 DNase (41.3)、淀粉酶 (41.3) 和脂肪酶 (32.6)。选定的分离株显示出对粪肠球菌的抗菌活性,并分离出Halomonas shengliensis和Idiomarina piscisalsi含有编码 dNDP-葡萄糖 4,6-脱水酶 (Glu 1) 的基因,表明它们具有生产具有脱氧糖部分的生物分子的潜力。棕榈酸或油酸是主要的脂肪酸,七个分离株含有一些多不饱和脂肪酸(亚麻酸或γ-亚麻酸)。有趣的是,属于Planococcus和Oceanobacillus属的6 个分离株产生了角鲨烯,一种生物活性类异戊二烯分子。在特比萘芬的存在下,它们的含量增加了 30-50%。潜在的生物活性和极端的生长条件使这种未开发的细菌多样性成为未来生物勘探研究的有希望的候选者。