Abstract

Danio rerio (zebrafish) is a suitable animal model that has been used to model a wide range of diseases such as aquaculture-relevant infectious agents and inflammatory processes. This study reviewed methods employed to study inflammation in zebrafish to reveal its usefulness in modeling inflammation. PubMed, Scopus, Web of Science, and Lilacs databases were searched for original research articles published until February 18, 2020, using the keywords “inflammation” AND “zebrafish.” Of the 3001 articles identified, 60 were included in this study. These included articles that compiled the methods used to induce inflammation with the most frequently used methodologies being tail fin injury and immersion in inflammatory solution. Moreover, the effectiveness of the methodology was confirmed by the increase in inflammatory cells (macrophages and neutrophils), enhanced levels of cytokines (TNF-α, IL-1β, IL-6, and IL-8), and chemokines (CXCL18-l1). These results confirmed that inflammatory processes in zebrafish are similar to those in other animal species, such as catfish (Ictalurus punctatus) and mammals. Therefore, zebrafish can be used to screen new anti-inflammatory agents and elucidate the pathway and pathology of inflammation, which can also be translated to other fishes and help to prevent fish diseases, resulting in a positive impact on the aquaculture economy.

摘要

斑马鱼（斑马鱼）是一种合适的动物模型，已被用于模拟各种疾病，例如与水产养殖相关的感染因子和炎症过程。本研究回顾了用于研究斑马鱼炎症的方法，以揭示其在模拟炎症方面的有用性。PubMed、Scopus、Web of Science 和 Lilacs 数据库使用关键词“炎症”和“斑马鱼”搜索了截至 2020 年 2 月 18 日发表的原创研究文章。在确定的 3001 篇文章中，有 60 篇被纳入本研究。其中包括汇编用于诱发炎症的方法的文章，其中最常用的方法是尾鳍损伤和浸泡在炎症溶液中。此外，炎症细胞（巨噬细胞和中性粒细胞）的增加证实了该方法的有效性，细胞因子（TNF-α、IL-1β、IL-6 和 IL-8）和趋化因子（CXCL18-l1）水平升高。这些结果证实斑马鱼的炎症过程与鲶鱼等其他动物物种的炎症过程相似。Ictalurus punctatus ) 和哺乳动物。因此，斑马鱼可用于筛选新的抗炎剂，阐明炎症的途径和病理，也可以转化为其他鱼类，有助于预防鱼类疾病，对水产养殖经济产生积极影响。