Abstract

This study analyzed the effects of the plant extracts (Citrus limon, Solanum lycopersicum, Zingiber officinale, Vitis vinifera and Allium sativum) on the growth of mammalian cells (Vero and MDA-MB-231) and evaluated the most effective plant extract for the expression of specific genes of the JAK/STAT pathway in human breast cancer cells. An antiproliferative bioassay involving neutral red-dye uptake was used to determine the anticancerous potential of plant extracts. In Vero cells, the ginger methanolic extract was least effective; whereas the lemon methanolic extract was more effective with 64 dilutions with IC₅₀ 51.42%. In MDA-MB-231 cells, the tomato and ginger methanolic, and grape water extracts were least effective, whereas lemon water extract was most effective with 32 dilutions with IC₅₀ 48.67%, by upregulating JAK1, JAK2, TYK2, IRF7 and IRF3 gene expressions of the JAK/STAT pathway. C. limon inhibited the growth of both Vero and MDA-MB 231 cells. It suggested that C. limon has anti-cancer potential by inducing the JAK/STAT pathway.

摘要

这项研究分析了植物提取物（柑橘，柠檬，茄子，姜，葡萄和大蒜）对哺乳动物细胞（Vero和MDA-MB-231）生长的影响，并评估了最有效的植物提取物的表达人乳腺癌细胞中JAK / STAT通路的特定基因的鉴定 使用涉及中性红染料摄取的抗增殖生物测定法来确定植物提取物的抗癌潜力。在Vero细胞中，姜汁的甲醇提取物效果最差。柠檬甲醇提取物用IC ₅₀稀释64倍更有效51.42％。在MDA-MB-231细胞中，番茄和姜汁的甲醇提取物和葡萄水提取物的效果最差，而柠檬水提取物在32种稀释度下（IC _50为48.67％）最有效，这是通过上调JAK1，JAK2，TYK2，IRF7和IRF3基因来实现的JAK / STAT通路的表达。柠檬衣原体同时抑制了Vero和MDA-MB 231细胞的生长。这表明柠檬柠檬假单胞菌通过诱导JAK / STAT途径具有抗癌潜力。