Cannabis extracts and products were analyzed by gas chromatography–mass spectrometry (GC–MS) with Cold EI for their full content including terpenes, sesquiterpenes, sesquiterpinols, fatty acids, delta 9‐tetrahydrocannabinol (THC), cannabidiol (CBD), other cannabinoids, hydrocarbons, sterols, diglycerides, triglycerides, and impurities. GC–MS with Cold EI is based on interfacing GC and MS with supersonic molecular beams (SMB) along with electron ionization of vibrationally cold sample compounds in the SMB in a fly‐through ion source (hence the name Cold EI). GC–MS with Cold EI improves all the performance aspects of GC–MS, enables the analysis of Cannabinoids with OH groups without derivatization, while providing enhanced molecular ions for improved identification, and enables internal quantitation without calibration. We found over 50 cannabinoid compounds including a new one with a Cold EI mass spectrum very similar to delta 9‐THC as well as relatively large cannabinoids with molecular weight above m/z = 400. Because the analysis was universal in full scan and not targeted, we found impurities such as bromo CBD and fluticasone propionate and could monitor the formation of oxidized CBD during decarboxylation. In addition, GC–MS with Cold EI enabled nontargeted full analysis of terpenes, sesquiterpenes, and sesquiterpinols in cannabis extracts with good internal quantitation. GC–MS with Cold EI further served with very good sensitivity for the concentration determination of delta 9‐THC in CBD‐related products. Finally, cannabis drugs such as EP‐1 used in Israel for treatment of epilepsy and for children with autism spectrum disorder (ASD) were analyzed for their full cannabinoids content for learning on the entourage effect and for drug activity optimization.

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大麻及其大麻素的气相色谱-质谱与冷 EI 分析

大麻提取物和产品通过气相色谱 - 质谱 (GC – MS) 和 Cold EI 分析其全部含量，包括萜烯、倍半萜烯、倍半萜醇、脂肪酸、δ 9-四氢大麻酚 (THC)、大麻二酚 (CBD)、其他大麻素，烃、甾醇、甘油二酯、甘油三酯和杂质。GC – MS with Cold EI 基于 GC 和 MS 与超音速分子束 (SMB) 的接口以及 SMB 中振动冷样品化合物在飞越离子源中的电子电离（因此得名 Cold EI）。带有 Cold EI 的 GC-MS 改进了 GC-MS 的所有性能方面，无需衍生即可分析带有 OH 基团的大麻素，同时提供增强的分子离子以改进鉴定，并无需校准即可进行内部定量。我们发现了 50 多种大麻素化合物，包括一种新的具有与 delta 9-THC 非常相似的冷 EI 质谱以及分子量高于 m/z = 400 的相对较大的大麻素。 ，我们发现了溴 CBD 和丙酸氟替卡松等杂质，可以监测脱羧过程中氧化 CBD 的形成。此外，带有 Cold EI 的 GC – MS 能够以良好的内部定量对大麻提取物中的萜烯、倍半萜烯和倍半萜醇进行非靶向全面分析。GC – MS 与 Cold EI 进一步为 CBD 相关产品中 delta 9-THC 的浓度测定提供了非常好的灵敏度。最后，