Pesticide contamination is an important factor in the global decline of amphibians. The herbicides glyphosate and 2,4-D are the most applied worldwide. These herbicides are often found in surface waters close to agricultural areas. This study aims at evaluating the chronic effects caused by glyphosate + 2,4-D mixture in Boana faber and Leptodactylus latrans tadpoles. The combined solution of the glyphosate and 2,4-D, in 5 different concentrations, was applied for 168 h. Herbicide mixtures did not affect the survival of the exposed tadpoles but growth and swimming activity were altered; besides causing several damages in the mouth and intestine. The erythrocytes showed micronuclei and other nuclear abnormalities. There is an ecological risk in the exposure of tadpoles of B. faber and L. latrans from the mixture of glyphosate + 2,4-D. Therefore, the approach used in this study provides important information on how commonly used pesticides can affect non-target organisms.

农药污染是全球两栖动物数量下降的一个重要因素。除草剂草甘膦和 2,4-D 是世界上应用最多的除草剂。这些除草剂通常存在于靠近农业区的地表水中。本研究旨在评估草甘膦 + 2,4-D 混合物对Boana faber和Leptodactylus latrans蝌蚪的慢性影响。将 5 种不同浓度的草甘膦和 2,4-D 的混合溶液施用 168 小时。除草剂混合物不影响暴露的蝌蚪的存活，但它们的生长和游泳活动发生了改变；除了对口腔和肠道造成一些伤害。红细胞显示微核和其他核异常。蝌蚪的暴露存在生态风险B. faber和L. latrans来自草甘膦 + 2,4-D 的混合物。因此，本研究中使用的方法提供了关于常用农药如何影响非目标生物的重要信息。