GATA1 is a key transcription factor in the GATA family, and promotes the differentiation and maturation of red blood cell, which is essential for normal hematopoiesis. Our results showed that the cDNA sequence of GATA1 was 2730 bp long encoding 443 amino acids. qRT-PCR analysis demonstrated that GATA1 had the highest expression in testis (T), followed by pituitary (P) and spleen (S). GATA1 gene expression in C. auratus red var. embryo from the neuroblast stage (N) to the embryo hatching (H) changes continuously; and the gene expression levels of nonylphenol (NP)-treated and those of control embryos were significantly different. Moreover, Methylation levels of GATA1 gene in NP-treated embryos were higher than those in control embryos, indicating that NP affected GATA1 methylation. Our study provides cues for further studying the roles of GATA1 gene in fish development, and suggested a potential molecular mechanism by which NP leads to abnormal development of fish embryos.

中文翻译：

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Car鱼红色变种GATA 1基因的克隆与表达分析

GATA1是GATA家族中的关键转录因子，可促进红细胞的分化和成熟，这对于正常的造血作用至关重要。我们的结果表明，GATA1的cDNA序列长2730 bp，编码443个氨基酸。qRT-PCR分析表明，GATA1在睾丸（T）中表达最高，其次是垂体（P）和脾（S）。GATA1基因表达在淡水梭菌红色变种中。从成神经细胞阶段（N）到胚胎孵化（H）的胚胎不断变化；壬基酚（NP）处理后的基因表达水平与对照组相比差异有统计学意义。此外，NP处理的胚胎中GATA1基因的甲基化水平高于对照胚胎，表明NP影响GATA1甲基化。