Quantitative diffuse reflectance spectroscopy (DRS) was developed for label-free, noninvasive, and real-time assessment of implanted tissue-engineered devices manufactured from primary human oral keratinocytes (six batches in two 5-patient cohorts). Constructs were implanted in a murine model for 1 and 3 weeks. DRS evaluated construct success in situ using optical absorption (hemoglobin concentration and oxygenation, attributed to revascularization) and optical scattering (attributed to cellular density and layer thickness). Destructive pre- and postimplantation histology distinguished experimental control from stressed constructs, whereas noninvasive preimplantation measures of keratinocyte glucose consumption and residual glucose in spent culture media did not. In constructs implanted for 1 week, DRS distinguished control due to stressed and compromised from healthy constructs. In constructs implanted for 3 weeks, DRS identified constructs with higher postimplantation success. These results suggest that quantitative DRS is a promising, clinically compatible technology for rapid, noninvasive, and localized tissue assessment to characterize tissue-engineered construct success in vivo.

中文翻译：

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原位植入组织工程构建成功的无创光学评估

定量漫反射光谱 (DRS) 用于无标记、无创和实时评估由原代人口腔角质形成细胞制造的植入式组织工程设备（两个 5 名患者队列中的六个批次）。将构建体植入鼠模型1和3周。DRS现场评估构建成功使用光吸收（血红蛋白浓度和氧合，归因于血运重建）和光散射（归因于细胞密度和层厚度）。破坏性植入前和植入后组织学将实验控制与压力构建体区分开来，而角质形成细胞葡萄糖消耗和用过的培养基中残留葡萄糖的无创植入前测量没有。在植入 1 周的构建体中，DRS 将控制与健康构建体的压力和损害区分开来。在植入 3 周的构建体中，DRS 确定了植入后成功率更高的构建体。这些结果表明，定量 DRS 是一种有前途的、临床兼容的技术，用于快速、无创和局部组织评估，以表征组织工程构建的成功在体内。