当前位置: X-MOL 学术Braz. J. Bot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
EST-SSR marker-based genetic diversity and population structure analysis of Indian Curcuma species: significance for conservation
Brazilian Journal of Botany ( IF 1.6 ) Pub Date : 2021-03-17 , DOI: 10.1007/s40415-021-00711-1
Ambika Sahoo , Sakuntala Behura , Subhashree Singh , Sudipta Jena , Asit Ray , Biswabhusan Dash , Basudeba Kar , Pratap Chandra Panda , Sanghamitra Nayak

Protection and utilization of plant genomic resources are greatly perceived these days. The possibility of their dwindling genetic diversity along with the augmented demands for the resources has necessitated immediate concern for their conservation and sustainable plant utilization. The genus Curcuma (family Zingiberaceae) is well known for its valuable multipurpose products for medicine, spices, food, fragrance, flavoring agents, condiments as well as ornamentals. Because of the resurgence of curiosity in the commercial development and conservation of Curcuma, it has become essential to precisely characterize the existing genetic diversity at the species level. In the present study, 109 EST-SSR markers showed cross-transferability among 9 Curcuma species, among which 33 showed polymorphism. These 33 polymorphic EST-SSRs were utilized to assess genetic resemblance and distance among Curcuma species. The results of cluster analysis and principal coordinate analysis highly correspond to each other. AMOVA showed higher genetic variation among Curcuma species. The population genetic study revealed the genetic differentiation of species. The mean values of Nei's gene diversity (H), Shannon's information index (I), polymorphic information content (PIC), percentage of polymorphic bands (PPB), and resolving Power (RP) were found to be 0.3314, 0.49, 0.66, 88.57% and 7.24, respectively. Values of Nei's gene diversity in C. longa indicated the need for its large-scale cultivation and conservation prioritization of wild taxa of C. caesia, C. aromatica and C. amada. These SSR markers could also be utilized to identify morphologically similar Curcuma species.



中文翻译:

基于EST-SSR标记的印度姜黄物种遗传多样性和种群结构分析:对保护的意义

这些天来,人们对植物基因组资源的保护和利用有了极大的认识。它们遗传多样性减少的可能性以及对资源的需求增加,已经迫切需要对其保护和可持续植物利用加以关注。属(家庭姜科)是众所周知的,其有价值的多用途产品用于医药,香料,食品,香精,调味剂,调味品以及观赏植物。由于好奇心在姜黄的商业开发和保护中重新兴起,因此在物种水平上精确表征现有遗传多样性已变得至关重要。在本研究中,109个EST-SSR标记在9个姜黄中显示出交叉转移性种,其中33种表现出多态性。论文利用33个多态性EST-SSRs来评估姜黄种间的遗传相似度和距离。聚类分析和主坐标分析的结果彼此高度对应。AMOVA表现出姜黄种之间较高的遗传变异。种群遗传学研究揭示了物种的遗传分化。Nei基因多样性(H),Shannon信息指数(I),多态信息含量(PIC),多态带百分比(PPB)和分辨力(RP)的平均值分别为0.3314、0.49、0.66 ,分别为88.57%和7.24。NEI的价值基因多样性C.姜黄指出需要大规模种植和保护优先级的凯西C. caesia)芳香族C.aromana)阿曼达(C.amada)。这些SSR标记也可用于鉴定形态相似的姜黄种类。

更新日期:2021-03-17
down
wechat
bug