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Crystal structure, DNA binding, cytotoxicity and anticancer ability of Zn(II) complex constructed by 2-(1,2,4)triazol-1-yl-isonicotinic acid
Inorganic Chemistry Communications ( IF 3.8 ) Pub Date : 2021-03-17 , DOI: 10.1016/j.inoche.2021.108571
Mingchang Zhu , Xiaoxi Ji , Songling Wang , Yi Zhou , Huahong Bao , Shuhan Li , Enjun Gao , Shuangyan Wu , Jing Wang , Qing Chen , Jin Xu , Xiaopeng Zhu

A novel Zn(II) complex was designed and synthesized under hydrothermal condition based on the ligand 2-(1,2,4)triazol-1-yl-isonicotinic acid. The crystal data were collected by X-ray single crystal diffractometer, and the purity of the complex was verified using elemental analysis and powder X-ray diffractometer. UV-Vis spectroscopy analyzed the binding mode of the complex and CT-DNA and calculated the binding constant Kb value of 3.36×103 M-1. Fluorescence spectroscopy further studied the interaction mode of the complex with CT-DNA, and the quenching constant Ksv value was 0.06. Gel electrophoresis was used to study the ability of the complex to cut pBR322 plasmid DNA. Flow cytometry detected the apoptosis ratio of the complex to HeLa cells, and the total apoptosis rate reached 71.4%. The fluorescent inverted microscope was used to observe the morphological changes of the cells by double staining, and detect the apoptotic effect on HeLa cells.



中文翻译:

2-(1,2,4)三唑-1-基-异烟碱酸构建的Zn(II)配合物的晶体结构,DNA结合,细胞毒性和抗癌能力

基于配体2-(1,2,4)三唑-1-基-异烟碱酸,在水热条件下设计合成了一种新型的Zn(II)配合物。用X射线单晶衍射仪收集晶体数据,并用元素分析和粉末X射线衍射仪验证络合物的纯度。紫外-可见光谱分析了复合物与CT-DNA的结合方式,计算出结合常数K b值为3.36×10 3 M -1。荧光光谱进一步研究了配合物与CT-DNA的相互作用模式以及猝灭常数K sv值为0.06。凝胶电泳用于研究复合物切割pBR322质粒DNA的能力。流式细胞仪检测复合物对HeLa细胞的凋亡率,总凋亡率达71.4%。荧光倒置显微镜通过双重染色观察细胞的形态变化,并检测其对HeLa细胞的凋亡作用。

更新日期:2021-03-17
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