BACKGROUND:Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue. OBJECTIVE:To develop and evaluate a novel, biologic antimicrobial coating for medical implants. METHODS:Electrochemically compacted collagen sheets with and withoutcrosslinked heparin were synthesized per protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release overtime from gentamicin infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC). RESULTS:Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not show any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was slow and remained above the minimal inhibitory concentration for gentamicin sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic payload within 24 hours. Overall, heparin associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC. CONCLUSION:We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.

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从电化学排列的胶原片中肝素介导的抗生素递送

背景：植入式医疗设备和硬件在医学领域多产，但硬件相关感染仍然是一个主要问题。目的：开发和评估一种用于医疗植入物的新型生物抗菌涂层。方法：根据我们小组制定的方案，合成含有和不含交联肝素的电化学压实胶原蛋白片。将片材在抗生素溶液（庆大霉素或莫西沙星）中孵育过夜，并通过针对铜绿假单胞菌的五天扩散试验评估体外活性。使用体外洗脱和高效液相色谱 (HPLC) 测定了庆大霉素输注片中抗生素释放的超时时间。结果：与单独的胶原-抗生素片相比，胶原-肝素-抗生素片对铜绿假单胞菌的生长抑制区更大。这种活动持续了五天，并且在评估之前不受冲洗纸的影响。冲洗过的胶原-抗生素片没有显示任何抑制区。从胶原-肝素-庆大霉素片中洗脱庆大霉素很慢，并在 29 天内保持在庆大霉素敏感生物的最小抑制浓度以上。相反，胶原蛋白-庆大霉素片在 24 小时内洗脱了它们的抗生素有效载荷。总体而言，肝素相关片显示出更大的对铜绿假单胞菌的抑制区和通过 HPLC 延长的洗脱曲线。结论：我们开发了一种新型的局部抗生素输送系统，可用于在未来涂覆医疗植入物/硬件并减少术后感染。冲洗过的胶原-抗生素片没有显示任何抑制区。从胶原-肝素-庆大霉素片中洗脱庆大霉素很慢，并在 29 天内保持在庆大霉素敏感生物的最低抑制浓度以上。相反，胶原蛋白-庆大霉素片在 24 小时内洗脱了它们的抗生素有效载荷。总体而言，肝素相关片显示出更大的对铜绿假单胞菌的抑制区和通过 HPLC 延长的洗脱曲线。结论：我们开发了一种新型的局部抗生素输送系统，可用于在未来涂覆医疗植入物/硬件并减少术后感染。冲洗过的胶原-抗生素片没有显示任何抑制区。从胶原-肝素-庆大霉素片中洗脱庆大霉素很慢，并在 29 天内保持在庆大霉素敏感生物的最低抑制浓度以上。相反，胶原蛋白-庆大霉素片在 24 小时内洗脱了它们的抗生素有效载荷。总体而言，肝素相关片显示出更大的对铜绿假单胞菌的抑制区和通过 HPLC 延长的洗脱曲线。结论：我们开发了一种新型的局部抗生素输送系统，可用于在未来涂覆医疗植入物/硬件并减少术后感染。相反，胶原蛋白-庆大霉素片在 24 小时内洗脱了它们的抗生素有效载荷。总体而言，肝素相关片显示出更大的对铜绿假单胞菌的抑制区和通过 HPLC 延长的洗脱曲线。结论：我们开发了一种新型的局部抗生素输送系统，可用于在未来涂覆医疗植入物/硬件并减少术后感染。相反，胶原蛋白-庆大霉素片在 24 小时内洗脱了它们的抗生素有效载荷。总体而言，肝素相关片显示出更大的对铜绿假单胞菌的抑制区和通过 HPLC 延长的洗脱曲线。结论：我们开发了一种新型的局部抗生素输送系统，可用于在未来涂覆医疗植入物/硬件并减少术后感染。