Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model,Bio-Medical Materials and Engineering

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Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model
Bio-Medical Materials and Engineering ( IF 1 ) Pub Date : 2021-03-23 , DOI: 10.3233/bme-201172
Shintaro Shoji ₁ , Kentaro Uchida _{1,

2} , Ryo Tazawa ₁ , Wataru Saito ₁ , Akiyoshi Kuroda ₁ , Hiroyuki Sekiguchi _{1,

2} , Daisuke Ishii ₁ , Sho Inoue ₁ , Gen Inoue ₁ , Masashi Takaso ₁

Affiliation

BACKGROUND:An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined. OBJECTIVE:We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. METHODS:C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. RESULTS:Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. CONCLUSIONS:IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.

中文翻译：

在小鼠临界尺寸骨缺损模型中使用含有骨形态发生蛋白 2 的原位形成的透明质酸水凝胶加速骨形成

背景：使用过氧化氢和辣根过氧化物酶的酶促交联策略在原位形成的水凝胶 (IFHG) 中的应用越来越受到关注。IFHGs 也可能是骨修复的理想载体材料，尽管它们携带骨形态发生蛋白 2 (BMP2) 的能力还有待研究。目的：我们研究了由含有 BMP2 的透明质酸 (IFHG-HA) 制成的 IFHG 在小鼠临界尺寸骨缺损模型中促进骨形成的有效性。方法：C57/BL6J 小鼠接受 2 毫米股骨临界尺寸骨缺损，然后被随机分配到以下治疗组之一（n = 6）：对照组（未治疗）、仅 IFHG-HA、PBS 和 BMP2，以及带有 BMP2 的 IFHG-HA。X 光片被用来追踪新骨的形成，术后两周对骨缺损部位形成的新骨进行显微计算机断层扫描和组织学检查。结果：用含 BMP2 的 PBS 和含 BMP2 的 IFHG-HA 处理的小鼠比接受对照的小鼠具有更大的骨体积 (BV) 和骨矿物质含量 (BMC)，并成功实现了巩固。用含有 BMP2 的 IFHG-HA 处理的小鼠比用含有 BMP2 的 PBS 处理的小鼠具有显着更高的 BV 和 BMC。结论：IFHG-HA 可能是 BMP2 的有效载体，可用于骨缺损修复。用含有 BMP2 的 IFHG-HA 处理的小鼠比用含有 BMP2 的 PBS 处理的小鼠具有显着更高的 BV 和 BMC。结论：IFHG-HA 可能是 BMP2 的有效载体，可用于骨缺损修复。用含有 BMP2 的 IFHG-HA 处理的小鼠比用含有 BMP2 的 PBS 处理的小鼠具有显着更高的 BV 和 BMC。结论：IFHG-HA 可能是 BMP2 的有效载体，可用于骨缺损修复。

更新日期：2021-03-27

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