Erwinia amylovora causes a quarantine disease called fire blight that affects most plants from the Rosaceae family. An efficient and rapid disease diagnosis tool is needed to prevent the spread of the pathogen. The main objective of this study was to develop a lateral flow immunoassay (LFIA) to detect E. amylovora and compare different organs of plants for optimization of LFIA testing. A total of 11 strains of E. amylovora and related species were tested for specificity of the developed LFIA. The detection limit of E. amylovora was equal to 4 × 10⁵ CFU mL⁻¹ in plant extracts. LFIA showed high specificity and did not demonstrate positive results with non-related species. Meanwhile, LFIA's effectiveness was confirmed through testing artificially infected leaf samples of apple, pear, and black raspberry. Reliable results were obtained 10 min after the start of LFIA for all testing strains. Different plant organs (121 samples) comprising apple, pear, hawthorn, quince, blackthorn, and cherry from naturally infected areas with symptoms of varying severity were tested. The LFIA was performed using samples from leaves, twigs, flowers, fruitlets, and bacterial ooze; for confirmation commercial kits based on fluorescent amplification–based specific hybridization PCR were used. Using several samples from one plant (cluster) significantly increased the accuracy of infected plant detection, the overlap of LFIA and PCR were equal to 70.2% for individual samples and 93.5% for clusters. Observed recovery of E. amylovora for different organs differed by up to 20%. We found out that using vascular tissues was better than using leaf extracts. This result demonstrates that LFIA's effectiveness improved when more appropriate samples were used.

淀粉欧文氏杆菌引起一种称为火疫病的隔离病，这种病会影响蔷薇科的大多数植物。需要一种有效且快速的疾病诊断工具来防止病原体扩散。这项研究的主要目的是开发一种侧向流免疫测定法（LFIA），以检测支链淀粉菌，并比较植物的不同器官以优化LFIA测试。总共测试了11种链球菌和相关种的发达LFIA的特异性。支链淀粉大肠埃希菌的检出限等于4×10 ⁵  CFU mL ^-1在植物提取物中。LFIA显示高特异性，未对非相关物种显示阳性结果。同时，LFIA的有效性通过测试人工感染的苹果，梨和黑莓的叶子样品得到了证实。LFIA开始后10分钟，所有测试菌株均获得了可靠的结果。测试了来自自然感染区域的苹果，梨，山楂，木瓜，黑刺李和樱桃等不同植物器官的严重程度不同的症状。LFIA是使用树叶，树枝，花朵，小果实和细菌渗出液中的样品进行的。为了进行确认，使用了基于基于荧光扩增的特异性杂交PCR的商业试剂盒。使用一棵植物（群集）中的多个样品可以显着提高被感染植物检测的准确性，LFIA和PCR的重叠对于单个样品而言等于70.2％，对于簇而言等于93.5％。观察到的恢复支链淀粉的不同器官差异最大为20％。我们发现使用维管组织比使用叶提取物更好。该结果表明，当使用更合适的样品时，LFIA的有效性得到了提高。