Visible-light driven CO₂ reduction to formate with the system consisting of water-soluble zinc tetraphneylporphyrin tetrasulfonate (ZnTPPS), formate dehydrogenase from Candida boidinii (CbFDH) and 1-nicotinamidethy-1’-methyl-4,4’- bipyridinium salt (NEMBP) in the presence of triethanolamine (TEOA) as an electron donor was investigated. NEMBP was prepared as the 4,4’-BP with the nicotinamide-group to promote the improvement of affinity with CbFDH. The properties of NEMBP were characterized by photochemical and electrochemical methods. In order to evaluate the affinity between cation radical of NEMBP (NEMBP^+.), as a co-enzyme and CbFDH, the energy level based on simple docking simulation and density functional theory were estimated. It was speculated that NEMBP^+. could bind near the substrate binding pocket of CbFDH as well as NAD⁺ by docking simulation. In the visible-light driven NEMBP reduction with the sensitization of ZnTPPS in the presence of TEOA, the yield for reduction of NEMBP to NEMBP^+. was estimated to be 80%. In the CO₂ reduction to formate with the system consisting of TEOA, ZnTPPS, NEMBP and CbFDH, the turnover numbers of NEMBP and CbFDH were estimated to be 0.14 and 1.53 h⁻¹, respectively.

中文翻译：

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在水溶性锌卟啉和甲酸脱氢酶系统中，可见光驱动的 CO2 还原为甲酸与电子介导的烟酰胺修饰的紫精

可见光驱动的 CO ₂还原为甲酸，该系统由水溶性四苯卟啉四磺酸锌 (ZnTPPS)、来自念珠菌的甲酸脱氢酶(CbFDH) 和 1-nicotinamidethy-1'-methyl-4,4'-bipyridinium 盐组成（ NEMBP) 在三乙醇胺 (TEOA) 作为电子供体的情况下进行了研究。NEMBP 被制备为具有烟酰胺基团的 4,4'-BP，以促进提高与 CbFDH 的亲和力。NEMBP 的性质通过光化学和电化学方法表征。为了评估作为辅酶的NEMBP阳离子自由基(NEMBP ^+. )与CbFDH之间的亲和力，基于简单对接模拟和密度泛函理论估计了能级。据推测，NEMBP^+。通过对接模拟，可以在 CbFDH 和 NAD ⁺的底物结合口袋附近结合。在可见光驱动的 NEMBP 还原中，在 TEOA 存在下，ZnTPPS 敏化，NEMBP 还原为 NEMBP ⁺的产率^。估计是80%。在用由 TEOA、ZnTPPS、NEMBP 和 CbFDH 组成的系统将 CO ₂还原为甲酸盐的过程中，NEMBP 和 CbFDH 的周转数估计分别为 0.14 和 1.53 h ^-1。