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Detection of DNA methylation in DBF1 gene of maize inbred W64A and mutant vp 14 exposed to drought stress
Cereal Research Communications ( IF 1.6 ) Pub Date : 2021-03-26 , DOI: 10.1007/s42976-021-00160-2
Nehal Sallam , Mounir Moussa , Mohamed Yacout , Huda M. Shakam

DNA methylation changes correlated with environmental stresses can confer a rapid adaptation to such challenges. Dehydration-responsive element-binding factor 1 (DBF1), a member of the AP2/EREBP transcriptional factor family, is induced by drought and salinity through an ABA-dependent pathway in maize seedlings. The present investigation was carried out to study the DNA methylation status of the DBF1 gene in two different maize genotypes, an inbred line W64A and a maize mutant vp14, by the methylation-sensitive restriction enzyme–polymerase chain reaction method. Stable DNA methylation upstream of the DBF1 gene in both W64A and vp14 was observed under non-stress and drought stress conditions; simultaneously, the methylation of particular cytosines in the proximal promoter or the transcribed regions was absent.



中文翻译:

玉米自交系W64A和干旱胁迫下的突变型vp 14的DBF1基因DNA甲基化的检测。

与环境压力相关的DNA甲基化变化可以赋予对此类挑战的快速适应能力。干旱和盐分通过玉米幼苗中的ABA依赖性途径诱导脱水响应元件结合因子1(DBF1),AP2 / EREBP转录因子家族的成员。本研究通过甲基化敏感的限制性内切酶-聚合酶链反应方法研究了两个不同基因型的玉米自交系W64A和玉米突变体vp14的DBF1基因的DNA甲基化状态。W64A和vp14中DBF1基因上游的稳定DNA甲基化在非胁迫和干旱胁迫条件下观察到;同时,在近端启动子或转录区域中不存在特定胞嘧啶的甲基化。

更新日期:2021-03-26
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