Journal of Environmental Science and Health, Part B ( IF 2 ) Pub Date : 2021-03-24 , DOI: 10.1080/03601234.2021.1900693 María A Marzocca 1 , Andrea R Costantino 2 , Alejandro R Gentili 1 , Alejandra S Oriani 1 , Marcelo T Pereyra 2 , Jorge Lusto 3 , Mónica D Baldini 1
Abstract
The aims of this research were 1) to find out the possible microbiological contamination and residues of pesticides (chlorpyrifos and cypermethrin) in lettuce grown in Bahía Blanca’s horticultural belt, and bacteriological quality of waters used for irrigation and post-harvest washing, 2) to investigate and compare the internalization capacity of a strain of Escherichia coli isolated from irrigation water with another hospital uropathogenic strain. Some strains of bacteria can internalize into the plant tissue. This capability presents food safety threats as they cannot be removed with standard sanitation practices. To determine bacteriological quality of lettuce, E. coli, Salmonella spp and E. coli O157: H7/NM (n = 10) were investigated. Bacteriological quality of water was determined by quantification of fecal coliforms/100 mL (n = 20). Pesticides were determined by using an improved and validated QuEChERS method combined with GC-MSD. Irrigation water in 19/20 samples did not present health risk. Bacteriological quality of lettuce met demands in 9/10 samples. Pesticides were detected in half of the samples, values exceeding the FAO recommendations. An effort is strongly recommended to implement both, best phytosanitary practices and monitoring programmes. Uropathogenic strain was able to internalize into plant tissue, while the nonpathogenic one was not.
中文翻译:
阿根廷巴伊亚布兰卡园艺带生产的生菜微生物质量及农药残留测定
摘要
本研究的目的是 1) 找出在 Bahía Blanca 园艺带种植的生菜中可能存在的微生物污染和农药(毒死蜱和氯氰菊酯)残留,以及用于灌溉和收获后清洗的水的细菌质量,2)调查并比较从灌溉水中分离的一株大肠杆菌与另一株医院尿路致病菌株的内化能力。一些细菌菌株可以内化到植物组织中。这种能力带来了食品安全威胁,因为它们无法通过标准卫生措施消除。确定生菜、大肠杆菌、沙门氏菌属和大肠杆菌的细菌学质量O157:研究了 H7/NM (n = 10)。水的细菌学质量通过粪便大肠菌群/100 mL (n = 20) 的定量确定。使用改进和验证的 QuEChERS 方法结合 GC-MSD 测定农药。19/20 样本中的灌溉水不存在健康风险。生菜的细菌学质量满足 9/10 样品的要求。在一半的样品中检测到农药,数值超过了粮农组织的建议。强烈建议努力实施最佳植物检疫措施和监测计划。泌尿病原体菌株能够内化到植物组织中,而非致病菌株则不能。