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NGS-based multiplex assay of trait-linked molecular markers revealed the genetic diversity of Iranian bread wheat landraces and cultivars
Crop & Pasture Science ( IF 1.9 ) Pub Date : 2021-03-24 , DOI: 10.1071/cp20362
Ehsan Rabieyan , Hadi Alipour

NGS-based multiplex assay has accomplished a valuable status as a means of high throughput research, rapid screening functional markers in wheat breeding programs. Accordingly, we applied a total of 42 locus-specific markers from Indel and SNP-mediated categorisations coupled with the agronomic important genes or quantitative trait loci (QTL) in bread wheat. The amplicons were analysed by an Ion Torrent Proton Sequencer. Then, an allele detection custom pipeline was applied to process the genotype of a total of 99 Iranian cultivars and 270 landraces. On the whole, 29 markers were positively incorporated and achieved 100% SNP call rates. Assessment of sequence-tagged site (STS) and competitive allele-specific PCR (KASP) markers concerning the same loci confirmed the genotype calls of all markers altogether. It was revealed that the Iranian cultivars and landraces supply a rich genetic resource capable of resisting Hessian fly, leaf rust, fusarium head blight, adult plant leaf diseases, stem rust, wheat soilborne mosaic virus, wheat streak mosaic, pre-harvest sprouting, high grain protein, and gluten strength traits. This finding can be developed to improve and enrich bread wheat. Further, it is advocated that NGS-based multiplex assay can be a promising approach for high throughput in examining trait-linked markers in wheat germplasm collections.



中文翻译:

基于NGS的性状相关分子标记的多重分析揭示了伊朗面包小麦地方品种和栽培品种的遗传多样性

基于NGS的多重测定已成为一种有价值的地位,可作为高通量研究手段,快速筛选小麦育种程序中功能标记的手段。因此,我们在面包小麦中应用了来自Indel和SNP介导的分类的总共42个基因座特异性标记,以及农艺学上的重要基因或数量性状基因座(QTL)。通过离子激流质子测序仪分析扩增子。然后,使用等位基因检测定制管道处理了总共99个伊朗品种和270个地方品种的基因型。总体而言,积极整合了29个标记,并实现了100%的SNP调用率。对涉及同一基因座的序列标记位点(STS)和竞争性等位基因特异性PCR(KASP)标记的评估证实了所有标记的基因型要求。据透露,伊朗的品种和地方品种提供了丰富的遗传资源,能够抵抗黑森州的苍蝇,叶锈病,镰刀菌枯萎病,成年植物叶病,茎锈病,小麦土传花叶病毒,小麦条纹花叶,收获前发芽,高谷物蛋白和面筋强度性状。这一发现可以发展为改良和丰富面包小麦。此外,提倡基于NGS的多重分析可能是一种有前途的方法,可用于检查小麦种质资源中性状相关标记的高通量。这一发现可以发展为改良和丰富面包小麦。此外,提倡基于NGS的多重分析可能是一种有前途的方法,可用于检查小麦种质资源中性状相关标记的高通量。这一发现可以发展为改良和丰富面包小麦。此外,提倡基于NGS的多重分析可能是一种有前途的方法,可用于检查小麦种质资源中性状相关标记的高通量。

更新日期:2021-03-25
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