Background: Ocimum basilicum L. seeds, is commonly also known as Takhmaria in Gujarat. It is a common constituent present in fruits, plant-derived beverages, vegetables, wheat, sprouts, and some seasonings.

Objective: A simple, specific, precise, accurate, and sensitive method for the quantification of apigenin by reverse-phase high-performance liquid chromatography (RP-HPLC) was developed and validated.

Methods: Analysis was carried out on Inertsil ODS-3V-C18 column (250 mm × 4.6 mm i.d, 5 μm) as stationary phase and methanol-acetonitrile (55:45 v/v) as a mobile phase at a flow rate of 1.0 mL/ min. Detection was carried out at 340 nm. The retention time of apigenin was found to be 8.30 min. The proposed method was validated according to ICH Guidelines, Q2 (R1).

Results: The developed method showed good linearity in the range of 10-50 μg/mL with a correlation coefficient (R2= 0.9998). The LOD and LOQ were found to be 1.23 μg/mL and 4.05 μg/mL, respectively. The percentage recovery for apigenin was found to be 97.75-100.5%. All validation parameters were found within acceptable limits and demonstrated good reliability in the quantification of apigenin.

Conclusion: Thus, the newly developed and validated method can be successfully applied for the quantification of apigenin from seeds of O. basilicum L. and can also be applied for the standardization of polyherbal formulation containing O. basilicum seeds. Assay results showed good recovery when statistically compared with the high-performance thin-layer chromatography (HPTLC) method.

背景：罗勒罗勒种子，在古吉拉特邦也俗称塔赫玛里亚。它是水果，植物饮料，蔬菜，小麦，豆芽和某些调味料中的常见成分。

目的：建立并验证了一种简便，特异性，精确，准确和灵敏的反相高效液相色谱（RP-HPLC）定量芹菜素的方法。

方法：以Inertsil ODS-3V-C18色谱柱（250 mm×4.6 mm内径，5μm）为固定相，以甲醇-乙腈（55:45 v / v）作为流动相，以1.0的流速进行分析毫升/分钟 在340nm进行检测。发现芹菜素的保留时间为8.30分钟。所提出的方法已根据ICH指南Q2（R1）进行了验证。

结果：所开发的方法在10-50μg/ mL的范围内显示出良好的线性，相关系数（R2 = 0.9998）。发现LOD和LOQ分别为1.23μg/ mL和4.05μg/ mL。发现芹菜素的回收率为97.75-100.5％。发现所有验证参数均在可接受的范围内，并且证明芹菜素的定量具有良好的可靠性。

结论：因此，新开发和验证的方法可以成功地用于定量从O. basilicum L.种子中提取的芹菜素，也可以用于标准化含有O. basilicum种子的多草药配方。与高效薄层色谱法（HPTLC）相比，从统计学角度分析测定结果显示出良好的回收率。