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Using high hydrostatic pressure as an abiotic elicitor strategy for improving capsaicin production in free and immobilized cell suspension cultures of Capsicum annuum L.
High Pressure Research ( IF 2 ) Pub Date : 2021-03-24 , DOI: 10.1080/08957959.2021.1903458
Cemil İşlek 1 , Esra Koç 2 , Ergin Murat Altuner 3 , Hami Alpas 4
Affiliation  

ABSTRACT

In this study, the effects of immobilization and high hydrostatic pressure (HHP) on capsaicin production in cell suspension culture of pepper seeds at different application times were investigated. Callus cultures were obtained from in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from calluses. Immobilized cell suspension cultures with calcium alginate and free cell suspension cultures were obtained by using these cell suspensions. 50, 100, 200, 300 and 400 MPa were applied to both free and immobilized cell suspensions as an elicitor. When all the results were compared, the highest amount of capsaicin was achieved in immobilized cell suspensions on the tenth day as 293.187 µg/g f.w at 400 MPa pressure. As a result, it can be concluded that the combined application of immobilization and elicitor (HHP) caused significant increases (p < .05) in the amount of capsaicin.



中文翻译:

使用高静水压作为一种非生物诱导剂策略来提高辣椒的游离和固定细胞悬浮培养物中辣椒素的产量。

摘要

在本研究中,研究了固定化和高静水压 (HHP) 对不同施用时间辣椒种子细胞悬浮培养中辣椒素产量的影响。从体外获得愈伤组织培养物从愈伤组织中制备辣椒幼苗的发芽下胚轴外植体和细胞悬液。通过使用这些细胞悬浮液,获得了具有海藻酸钙的固定化细胞悬浮培养物和游离细胞悬浮培养物。将 50、100、200、300 和 400 MPa 作为激发剂应用于游离和固定细胞悬液。比较所有结果时,在 400 MPa 压力下,第十天固定化细胞悬液中辣椒素的含量最高,为 293.187 µg/g fw。因此,可以得出结论,固定化和诱导剂 (HHP) 的联合应用导致 辣椒素量显着增加 ( p < .05)。

更新日期:2021-06-02
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