ABSTRACT

The selection of fast-growing and high-yield-producing strains is required to satisfy the market demand on fungal food supplements. To that aim, three strains deposited in our collection as G. lucidum and G. oregonense were screened for polysaccharide production and biomass yield. Ganoderma strains deposited as G. lucidum were identified as G. sessile and G. lingzhi by nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS) and translation elongation factor 1-α (TEF1-α) phylogenies. The identity of G. oregonense was confirmed by molecular phylogeny and biogeography. Additionally, mycelial antagonism confirmed species differentiation, and strains were further distinguished by morphology and protein profiles. Biomass and polysaccharide yields of G. sessile were clearly different from those of G. lingzhi and G. oregonense in both liquid culture and solid-state fermentation. The maximum polysaccharide yield (4.52 ± 0.83 g L⁻¹) for G. sessile was obtained from submerged cultures at day 9. G. sessile also achieved the highest linear growth in lignocellulosic solid substrates. Consequently, basidiomata were successfully obtained by solid-state fermentation in polypropylene bags, whereas G. lingzhi and G. oregonense mushrooms were not produced in artificial solid substrates. G. sessile, a species frequently collected in America, showed to be a promising polysaccharide producer for the manufacture of dietary supplements.

摘要

需要选择生长速度快、产量高的菌株，以满足市场对真菌食品补充剂的需求。为此，我们针对多糖产量和生物量产量筛选了作为G. lucidum和G. oregonense存放在我们的集合中的三个菌株。通过 nuc rDNA 内部转录间隔区 ITS1-5.8S-ITS2 (ITS) 和翻译延伸因子 1-α ( TEF1-α ) 系统发育，将作为G. lucidum保藏的灵芝菌株鉴定为G. sessile和G. lingzhi。G. oregonense的身份被分子系统发育和生物地理学证实。此外，菌丝体拮抗作用证实了物种分化，并且通过形态学和蛋白质谱进一步区分了菌株。G. sessile 的生物量和多糖产量与G. lingzhi和G. oregonense在液体培养和固态发酵中明显不同。G. sessile的最大多糖产量 (4.52 ± 0.83 g L ^-1 )在第 9 天从浸没的培养物中获得。G. sessile在木质纤维素固体基质中也实现了最高的线性生长。因此，担子在聚丙烯袋中通过固态发酵成功获得，而G. lingzhi和G. oregonense蘑菇不是在人造固体基质中产生的。G. sessile是一种经常在美国采集的物种，显示出是用于制造膳食补充剂的有前途的多糖生产者。