Interleukin (IL)-1 plays a key role in carcinogenesis, tumor progression, and metastasis. Although IL-1 may enhance the expansion of CD8+ T-cells, the pathological contribution of IL-1-activated CD8+ T-cells to tumor metastasis remains unclear. This study used a liver metastasis model of the EL4 T-cell lymphoma cells transplanted into human IL (hIL)-1α conditional transgenic (hIL-1α cTg) mice. Overproduction of hIL-1α suppressed both macroscopic and histological liver metastasis of EL4 T-cell lymphoma. The hIL-1α-induced inflammatory state increased the number of CD8+ T-cells both within and around metastatic tumors. Moreover, larger numbers of CD8+ T-cells showed greater infiltration of liver blood vessels in hIL-1α cTg mice than in control wild-type mice. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining of liver tissue from hIL-1α cTg mice indicated increased apoptosis of cells in the tumor. Localization of apoptosis cells resembled that of CD8+ T-cells. In addition, cytotoxicity assay showed that CD8+ T-cell counts from tumor-bearing hIL-1α cTg mice correlated with cytotoxicity against EL4. In summary, IL-1α suppresses lymphoma metastasis, and IL-1α-activated CD8+ T-cells may play important roles in inhibiting both tumor metastasis and metastatic tumor growth:

白介素（IL）-1在致癌，肿瘤进展和转移中起关键作用。尽管IL-1可能增强CD8 + T细胞的扩增，但IL-1激活的CD8 + T细胞对肿瘤转移的病理学贡献仍不清楚。这项研究使用了移植到人IL（hIL）-1α条件性转基因（hIL-1αcTg）小鼠中的EL4 T细胞淋巴瘤细胞的肝转移模型。hIL-1α的过量生产抑制了EL4 T细胞淋巴瘤的宏观和组织学肝转移。hIL-1α诱导的炎症状态增加了转移性肿瘤内部和周围的CD8 + T细胞数量。此外，与对照野生型小鼠相比，hIL-1αcTg小鼠中大量的CD8 + T细胞显示出更大的肝血管浸润。hIL-1αcTg小鼠肝脏组织的末端脱氧核苷酸转移酶dUTP缺口末端标记染色表明肿瘤细胞的凋亡增加。凋亡细胞的定位类似于CD8 + T细胞的定位。此外，细胞毒性试验表明，来自荷瘤hIL-1αcTg小鼠的CD8 + T细胞计数与针对EL4的细胞毒性相关。总之，IL-1α抑制淋巴瘤转移，而IL-1α激活的CD8 + T细胞可能在抑制肿瘤转移和转移性肿瘤生长中起重要作用：