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Influence of different hydrophilic interaction liquid chromatography stationary phases on method performance for the determination of highly polar anionic pesticides in complex feed matrices
Journal of Separation Science ( IF 3.1 ) Pub Date : 2021-03-24 , DOI: 10.1002/jssc.202001134
Jonatan Dias 1 , Sonia Herrera López 1 , Hans Mol 1 , André de Kok 1
Affiliation  

Hydrophilic interaction liquid chromatography is an alternative liquid chromatography mode for separation of polar compounds. In the recent years, this liquid chromatography mode has been recognized as an important solution for the analysis of compounds not amenable to reverse phase chromatography. In this work, we evaluated three different hydrophilic liquid chromatography stationary phases for the determination of 14 highly polar anionic molecules including pesticides such as glyphosate, glufosinate, ethephon and fosetyl, their main metabolites, and bromide, chlorate, and perchlorate. Several mobile phase compositions were evaluated combined with different gradients for the chromatographic run. The two columns that presented the best results were used to assess the performance for the determination of the 14 compounds in challenging highly complex feed materials. Very different matrix effects were observed for most of the compounds in each column, suggesting that different interactions can occur. Using isotopically labeled internal standards, acceptable quantitative performance and identification could be achieved down to 0.02 mg kg−1 (the lowest level tested) for most compounds. While one column was found to be favorable in terms of scope (suited for all 14 compounds), the other one was more suited for quantification and identification at lower levels, however, not for all analytes tested.

中文翻译:

不同亲水相互作用液相色谱固定相对复杂进料基质中高极性阴离子农药测定方法性能的影响

亲水相互作用液相色谱是分离极性化合物的另一种液相色谱模式。近年来,这种液相色谱模式已被公认为分析不适合反相色谱的化合物的重要解决方案。在这项工作中,我们评估了三种不同的亲水液相色谱固定相,用于测定 14 种高极性阴离子分子,包括草甘膦、草铵膦、乙烯利和二甲磷等农药及其主要代谢物,以及溴化物、氯酸盐和高氯酸盐。结合不同梯度的色谱运行评估了几种流动相组成。使用呈现最佳结果的两根色谱柱来评估在具有挑战性的高度复杂进料中测定 14 种化合物的性能。对于每根色谱柱中的大多数化合物,观察到非常不同的基质效应,表明可能发生不同的相互作用。使用同位素标记的内标,可以实现可接受的定量性能和鉴定,低至 0.02 mg kg-1(测试的最低水平)对于大多数化合物。虽然发现一根色谱柱在范围方面是有利的(适用于所有 14 种化合物),但另一根色谱柱更适用于较低水平的定量和鉴定,但并非适用于所有测试的分析物。
更新日期:2021-03-24
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