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Applications of electrochemical biosensor of aptamers-based (APTASENSOR) for the detection of leukemia biomarker
Sensing and Bio-Sensing Research Pub Date : 2021-03-23 , DOI: 10.1016/j.sbsr.2021.100416
Yuspian Nur , Shabarni Gaffar , Yeni Wahyuni Hartati , Toto Subroto

Leukemia is a deadly cancer disease, and according to World Health Organization (WHO) data, there were 437,033 cases across the world in 2018. Early detection of this cancer is very difficult, as its symptoms are hidden and clinically invisible. Conventional leukemia detection methods are very costly and time-consuming, so a simple, sensitive, and rapid method is needed. The electrochemical biosensor is an alternative method. Bioreceptor modification has been shown to increase the performance of the biosensors. Currently, Aptamers are potential molecule probes with which to diagnose the development of leukemia. The selected aptamers are obtained by Systematic Evolution of Ligands by Exponential enrichment (SELEX) method. Aptamers are excellent compared to antibodies, as they have low toxicity, are easy to modify, offer high stability, can easily penetrate tissues, and have a low production cost. Furthermore, the technology of aptamers as biosensor was selective process on the unique cells. It has high affinity, selectivity, and sensitivity when bound specifically with a molecule or biomarker. Aptamers have dissociation constant which is in the range of nM to pM. This review summarizes the aptamers aptamers type for leukemia and the development of leukemia diagnosis using the electrochemical aptamers-based biosensor. Finally, we discuss the challenges and potential applications of aptamers as leukemia aptasensor.



中文翻译:

基于适体的电化学生物传感器(APTASENSOR)在白血病生物标志物检测中的应用

白血病是一种致命的癌症疾病,根据世界卫生组织(WHO)的数据,2018年全球有437,033例病例。这种癌症的症状是隐藏的且在临床上不可见,因此很难尽早发现。常规的白血病检测方法非常昂贵且费时,因此需要一种简单,灵敏且快速的方法。电化学生物传感器是一种替代方法。已显示生物受体修饰可提高生物传感器的性能。目前,适体是诊断白血病发展的潜在分子探针。通过配体通过指数富集的系统进化(SELEX)方法获得选择的适体。与抗体相比,适体具有优异的毒性,因为它们毒性低,易于修饰,稳定性高,容易渗透组织,生产成本低。此外,适体作为生物传感器的技术是对独特细胞的选择性过程。当与分子或生物标记物特异性结合时,它具有高亲和力,选择性和敏感性。适体具有在nM至pM范围内的解离常数。这篇综述总结了用于白血病的适配子类型以及使用基于电化学适配子的生物传感器对白血病的诊断发展。最后,我们讨论了适体作为白血病适体传感器的挑战和潜在应用。适体具有在nM至pM范围内的解离常数。这篇综述总结了用于白血病的适配子类型以及使用基于电化学适配子的生物传感器对白血病的诊断发展。最后,我们讨论了适体作为白血病适体传感器的挑战和潜在应用。适体具有在nM至pM范围内的解离常数。这篇综述总结了用于白血病的适配子类型以及使用基于电化学适配子的生物传感器对白血病的诊断发展。最后,我们讨论了适体作为白血病适体传感器的挑战和潜在应用。

更新日期:2021-03-27
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