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Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane
Royal Society Open Science ( IF 3.5 ) Pub Date : 2021-03-24 , DOI: 10.1098/rsos.201618
Tanapong Watchararot 1 , Weerapong Prasongchean 1 , Peerapat Thongnuek 2, 3, 4
Affiliation  

Angiogenesis is a crucial step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with the porosity of 77.34 ± 6.96% and the pore diameter of 513.95 ± 4.99 µm were implanted on the CAM of chick embryos that were on an embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, the ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. By contrast, the control group could not induce the same extent of angiogenesis. In vitro cytotoxicity tests and in vivo angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerate angiogenesis, and hence they can be a promising biomaterial for the regeneration of tissues that require angiogenesis.



中文翻译:

丝素蛋白支架与脂肪干细胞在鸡绒膜尿囊膜上的血管生成特性

血管生成是组织再生和修复中的关键步骤。因此,允许或促进血管生成的生物材料是有益的。在这项研究中,以鸡绒毛尿囊膜(CAM)为模型,研究了接种人脂肪干细胞(hADSCs)的盐浸丝素蛋白(SF)支架的血管生成特性。将hADSC种子植入的SF支架(SF-hADSC)的孔隙率为77.34±6.96%,孔径为513.95±4.99 µm,植入了处于胚胎发育第8天(E8)的雏鸡的CAM。SF-hADSC支架诱导了毛细血管网络的辐条轮模式,指示了血管新生,这自E11以来就很明显。此外,在组织学切片中观察到血管向支架内的向内生长。未播种的支架随后在E14上诱导了相同程度的血管生成。相比之下,体外细胞毒性试验和体内血管刺激性研究重申了支架的生物相容性。这项工作强调了生物相容性SF-hADSC支架可以加速血管生成,因此它们可以成为有希望的生物材料用于需要血管生成的组织再生。

更新日期:2021-03-24
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