Family with sequence similarity 84, member B (FAM84B) has recently emerged as an oncoprotein in multiple types of cancer. However, whether FAM84B modulates the progression of glioma has not been determined. The goals of this work were to assess the possible relationship between FAM84B and glioma. Our data revealed high FAM84B level in glioma specimens and exhibited that the overexpression of FAM84B was correlated with a low survival rate in glioma patients. Cellular functional assays showed that silencing of FAM84B prohibited the proliferation and invasion, and induced the apoptosis of glioma cells. Further results determined that the knockdown of FAM84B remarkably decreased the levels of phosphorylated Akt and glycogen synthase kinase (GSK)-3β, and active β-catenin. Inhibition of Akt abolished the FAM84B-mediated promotion effects on Wnt/β-catenin pathway. The subcutaneous xenograft assay confirmed that the silencing of FAM84B significantly prohibited the tumorigenicity of glioma cells in vivo. Collectively, the findings from this work demonstrate that the downregulation of FAM84B exhibits a cancer-suppressive role in human glioma through the regulation of Akt/GSK-3β/β-catenin pathway.

中文翻译：

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FAM84B 通过影响 Akt/GSK-3β/β-catenin 通路作为人类胶质瘤的肿瘤启动子

具有序列相似性 84 的家族成员 B (FAM84B) 最近已成为多种癌症中的一种癌蛋白。然而，尚未确定 FAM84B 是否调节神经胶质瘤的进展。这项工作的目的是评估 FAM84B 与胶质瘤之间可能存在的关系。我们的数据显示胶质瘤标本中的 FAM84B 水平较高，并表明 FAM84B 的过表达与胶质瘤患者的低存活率相关。细胞功能测定表明，FAM84B 的沉默抑制了胶质瘤细胞的增殖和侵袭，并诱导了胶质瘤细胞的凋亡。进一步的结果确定，FAM84B 的敲低显着降低了磷酸化 Akt 和糖原合酶激酶 (GSK)-3β 以及活性 β-连环蛋白的水平。Akt 的抑制消除了 FAM84B 介导的对 Wnt/β-catenin 通路的促进作用。皮下异种移植试验证实，FAM84B 的沉默显着抑制了体内胶质瘤细胞的致瘤性。总的来说，这项工作的结果表明，FAM84B 的下调通过调节 Akt/GSK-3β/β-catenin 通路在人类胶质瘤中表现出抑癌作用。