Halogeton glomeratus (H. glomeratus) is one of the most important halophytes in Asia, and the research on the genes and salt-tolerant mechanisms of this species is limited because of the lack of an optimal and efficient in vitro regeneration system. Here, we developed an efficient plant regeneration protocol using H. glomeratus leaves as explants, which has not been previously reported. High-quality calli were successfully obtained from the H. glomeratus leaves at a frequency of 100% supplemented with 2.00 mg/L 2,4-dichlorophenoxyacetic acid, 0.50 g/L PVP, 5.00 g/L agar, and 30.00 g/L sucrose; the optimum callus subculture medium is 1.00 mg/L 2,4-dichlorophenoxyacetic acid, 0.50 g/L PVP, 5.00 g/L agar, and 30.00 g/L sucrose. Shoots were regenerated on shoot induction medium at a frequency of 100% added with 0.50 mg/L 6-benzylaminopurine, 2.00 mg/L kinetin, 0.20 mg/L naphthaleneacetic acid, 0.50 g/L PVP, 5.00 g/L agar, and 30.00 g/L sucrose; then, shoots grew up on shoot regeneration medium. Finally, roots were regenerated from the shoots on Murashige and Skoog medium with high efficiency. This study firstly offers a rapid and efficient system for plantlet regeneration from leaves of H. glomeratus. Whether our protocol applies well to other tissue regeneration of H. glomeratus needs further confirmation. This work will facilitate basic research and salt-tolerant mechanisms of this important halophyte species.

Halogeton glomeratus（H. glomeratus）是亚洲最重要的盐生植物之一，由于缺乏最佳和有效的体外再生系统，对该物种的基因和耐盐机制的研究受到限制。在这里，我们开发了一种有效的植物更新协议，该方法使用之前的报道尚未发现有使用球形小球藻叶作为外植体的植物。从H. glomeratus成功获得了高质量的愈伤组织叶片以100％的频率补充2.00 mg / L的2,4-二氯苯氧基乙酸，0.50 g / L的PVP，5.00 g / L的琼脂和30.00 g / L的蔗糖; 最佳的愈伤组织继代培养基是1.00 mg / L 2,4-二氯苯氧基乙酸，0.50 g / L PVP，5.00 g / L琼脂和30.00 g / L蔗糖。在芽诱导培养基上以100％的频率再生芽，添加0.50 mg / L的6-苄氨基嘌呤，2.00 mg / L的激动素，0.20 mg / L的萘乙酸，0.50 g / L的PVP，5.00 g / L的琼脂和30.00 g / L蔗糖；然后，新芽在新芽再生培养基上长大。最后，从Murashige和Skoog培养基上的芽再生出根，效率很高。这项研究首先提供了一种快速有效的系统，用于从球果H. glomeratus的叶片中再生苗。。我们的协议是否适用于肾小球菌的其他组织再生还需要进一步证实。这项工作将促进这一重要盐生植物物种的基础研究和耐盐机制。