2D-HPLC is an important development in chiral separations due to its excellent capability to resolve the enantiomers. This article describes the latest update in the chiral resolution of racemates using 2D-HPLC. The various aspects of the chiral resolution such as validation, optimization, and applications are discussed in both biological and environmental matrices. Some latest developments of nano-2D-HPLC are also included. Besides, efforts are made to discuss the challenges and future perspectives. During the preparation of this article, it was observed that much work has not been done in this direction and only a few papers describe the application of 2D-HPLC in the chiral separation. There is an urgent need to develop more 2D-HPLC methods for a variety of racemates. This article will be useful to the investigators, academicians and industry persons in the future to improve 2D-HPLC for the chiral resolution of the various racemates.

2D-HPLC由于其出色的拆分对映异构体的能力，是手性分离领域的重要发展。本文介绍了使用2D-HPLC进行外消旋物手性拆分的最新更新。在生物和环境基质中都讨论了手性拆分的各个方面，例如验证，优化和应用。纳米2D-HPLC的一些最新进展也包括在内。此外，还努力讨论了挑战和未来前景。在本文的制备过程中，观察到在此方向上尚未完成许多工作，只有几篇论文描述了2D-HPLC在手性分离中的应用。迫切需要为各种外消旋物开发更多的2D-HPLC方法。本文将对研究人员有用，