Microalgal biotechnology has increased rapidly owing to have high value bioactive compounds and numerous consumer products that can be utilized from microalgae. With the development of novel cultivation and processing methods, microalgal biotechnology can meet the high demands of food, energy and pharmaceutical industries. In this context, especially for food and pharmaceutical applications, encapsulation of microalgal bioactive compounds is carried out to protect the compound from oxidation and degradation. In this study, a microalgal production process was carried out and microalgal oil loaded bovine serum albumin (BSA) nanoparticle production using glucose as cross-linking agent was investigated. The influences of different process parameters such as initial BSA concentration, glucose concentration and desolvation temperature on the size of BSA nanoparticles were investigated to achieve very small size nanoparticles. Furthermore, data obtained from the experiments were assessed statistically to model the process. It was found that the obtained nanoparticles showed spherical shape with the mean particle size of around 200–300 nm with zeta potential of about − 23 mV. Also, stability test showed that, there was not any change in particle size for one month storage and nanoparticle structure enhance the protection of microalgae oil from oxidation. At last, antibacterial effect of nanoparticles was presented against E. coli ATCC 8739 and L. monocytogenes ATCC 13932. In here, we demonstrated a microalgal bioprocess which consists of microalgae production to obtain microalgal oil riched in bioactive and, encapsulation of microalgal oil to protect it from environmental conditions.

由于具有高价值的生物活性化合物和可从微藻中利用的众多消费品，微藻生物技术迅速发展。随着新型栽培和加工方法的发展，微藻生物技术可以满足食品、能源和制药行业的高要求。在这种情况下，特别是对于食品和药物应用，微藻生物活性化合物的封装是为了保护化合物免受氧化和降解。在这项研究中，进行了微藻生产过程，并研究了使用葡萄糖作为交联剂的微藻油负载牛血清白蛋白 (BSA) 纳米颗粒的生产。不同工艺参数的影响，如初始 BSA 浓度、研究了葡萄糖浓度和去溶剂化温度对 BSA 纳米颗粒尺寸的影响，以获得非常小的纳米颗粒。此外，对从实验获得的数据进行统计评估以模拟该过程。发现获得的纳米颗粒呈球形，平均粒径约为 200-300 nm，zeta 电位约为 - 23 mV。此外，稳定性测试表明，储存1个月后粒径没有任何变化，纳米颗粒结构增强了微藻油的抗氧化保护。最后展示了纳米粒子的抗菌作用 发现获得的纳米颗粒呈球形，平均粒径约为 200-300 nm，zeta 电位约为 - 23 mV。此外，稳定性测试表明，储存1个月后粒径没有任何变化，纳米颗粒结构增强了微藻油的抗氧化保护。最后展示了纳米粒子的抗菌作用 发现获得的纳米颗粒呈球形，平均粒径约为 200-300 nm，zeta 电位约为 - 23 mV。此外，稳定性测试表明，储存1个月后粒径没有任何变化，纳米颗粒结构增强了微藻油的抗氧化保护。最后展示了纳米粒子的抗菌作用大肠杆菌ATCC 8739 和单核细胞增生李斯特菌ATCC 13932。在这里，我们展示了一种微藻生物过程，该过程包括微藻生产以获得富含生物活性的微藻油，并封装微藻油以保护其免受环境条件的影响。