Fungi are considered to be the most resilient and economically important microbial community that can easily survive and optimally grow under a wide range of growth conditions. Thermophilic fungi from the geothermal sources have been less pondered upon and lie unexplored. Here, a microbiome approach was conducted to understand the concealed world of the environmental mycobiota from the two hot springs of North Sikkim district located in North-east India. The solfataric muds from the hot springs were analyzed. In both the samples, on the basis of genus level classification, genus Fusarium had the highest abundance followed by Colletotrichum, Pochonia, Pyricularia, Neurospora, etc. Analyzing the predicted genes, the functional proteins of New Yume Samdung mycobiome were found to be dominated by the genera Fusarium (22%), Trichoderma (12%), and Aspergillus (11%), whereas in the case of Old Yume Samdung, it was dominated by the genera Aspergillus (11%), Saccharomyces (6%), and Fusarium (5%). Interestingly, in the studied mycobiome, environmental yeasts were also detected. From the functional metagenomics, sulfate adenylatetransferase (SAT) proteins for sulfur assimilation were found in some of the fungal reads. Toxin protein reads such as AM-toxin biosynthesis proteins, AF-toxin biosynthesis proteins, Gliotoxin biosynthesis proteins, and aflatoxin biosynthesis proteins were detected in the mycobiomes.

真菌被认为是最具弹性和经济上最重要的微生物群落，可以在各种生长条件下轻松生存和最佳生长。来自地热源的嗜热真菌很少被考虑并且未被探索。在这里，通过微生物组方法来了解位于印度东北部的北锡金地区的两个温泉中环境真菌群的隐蔽世界。分析了温泉中的溶胶泥。在这两个样本，属一级分类的基础上，属枯萎了的丰度最高其次是炭疽病，Pochonia，稻瘟病，链孢霉等分析预测基因，发现New Yume Samdung 菌群的功能蛋白以镰刀菌属（22%）、木霉属（12%）和曲霉属（11%）为主，而在Old Yume 的情况下Samdung，它以曲霉属（11%）、酵母菌（6%）和镰刀菌属为主(5%)。有趣的是，在研究的真菌群落中，也检测到环境酵母菌。从功能宏基因组学中，在一些真菌读数中发现了用于硫同化的硫酸腺苷酸转移酶 (SAT) 蛋白。在真菌生物组中检测到毒素蛋白读数，例如 AM-毒素生物合成蛋白、AF-毒素生物合成蛋白、Gliotoxin 生物合成蛋白和黄曲霉毒素生物合成蛋白。